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Hippocampal single-nucleus sequencing identifies a unique transcriptional signature that is predictive of reactivity in dentate granule neurons

ABSTRACT: Neuronal circuits are constantly remodeled in response to activity, but the extent and the specificity of activity-induced transcription are unknown. In this study, we used single nucleus RNA-seq to compare transcriptional responses to activity throughout the hippocampus following exposure to a novel environment. By comparing activated and non-activated nuclei, we found that dentate granule cells (DGCs) have a unique transcriptional response to activity that differs in both magnitude and quality compared to CA1 pyramidal cells and vasoactive intestinal polypeptide (VIP) interneurons. We further used single nuclei RNA-seq to compare transcriptional responses to activity throughout the dentate gyrus following exposure to two contexts and identified that DGCs generate a transcriptional signature that is predictive of reactivity.

ORGANISM(S): Mus musculus

PROVIDER: GSE98679 | GEO | 2018/07/27

REPOSITORIES: GEO

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Neuronal activity-related transcription is blunted in immature compared to mature dentate granule cells

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