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Analysis of genes regulated by IKKb (Ird5) in the presence or absence of CrPV (Cricket paralysis virus) infection in Drosophila S2 cells

ABSTRACT: This DNA microarray analysis (agilent) helps to identify candidate genes regulated by IKKb (Ird5) on Non-infected condition and after CrPV infection (2h and 6h).

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE99044 | GEO | 2018/08/14

REPOSITORIES: GEO

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Analysis of IKKb (Ird5) regulated genes in control and hemizygote IKKb (Ird5) null mutant adult flies after DCV (Drosophila C virus) infection

Project description:This DNA microarray analysis (agilent) help to identify candidate genes induced by DCV infection and regulated by IKKb (Ird5) in control (Df/+) and hemizygote IKKb (Ird5) null mutant (Dr/Ird5[1]) adult flies.

2018-08-14 | GSE99043 | GEO

Compensatory IKKa activation of classical NF-kB signaling during IKKb inhibition

Project description:A subtype of diffuse large B-cell lymphoma (DLBCL), termed activated B-cell-like (ABC) DLBCL, depends on constitutive NF-kB pathway signaling for survival. Small molecule inhibitors of IkB kinase b (IKKb), a key regulator of the NF-kB pathway, kill ABC DLBCL cells and hold promise for the treatment of this lymphoma type. We conducted an RNA interference genetic screen to investigate potential mechanisms of resistance of ABC DLBCL cells to IKKb inhibitors. We screened a library of small hairpin RNAs (shRNAs) targeting 500 protein kinases for shRNAs that would kill an ABC DLBCL cell line in the presence of a small molecule IKKb inhibitor more effectively than in its absence. Two independent shRNAs targeting IKKa synergized with the IKKb inhibitor to kill three different ABC DLBCL cell lines but were not toxic by themselves. Surprisingly, IKKa shRNAs blocked the classical rather than the alternative NF-kB pathway in ABC DLBCL cells, as judged by inhibition of IkBa phosphorylation. IKKa shRNA toxicity was reversed by coexpression of wild type but not kinase inactive forms of IKKa, suggesting that IKKa may directly phosphorylate IkBa under conditions of IKKb inhibition. These results suggest that therapy for ABC DLBCL may be improved by targeting both IKKa and IKKb. Keywords: compound treatment design Gene expression profiling of OCI-Ly3 cells with or without expressing IKKa shRNA in the presence or absence of 12.5 uM IKKb inhibitor for 2 and 3 days. Four samples were analyzed.

2009-06-01 | E-GEOD-11902 | biostudies-arrayexpress

Effect of tubular IKKb deletion in acute ischemic kidney injury

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2022-07-09 | GSE207430 | GEO

Expression profile of WT and IKKb(EE)IEC epithelial cells (IEC)

Project description:To investigate NF-kB-driven gene expression in IEC, we performed microarray analysis from enterocytes of mice that express a constitutively active form of IKKb in intestinal epithelial cells Total RNA was prepared from enterocytes obtained from small intestines of IKKb(EE)IEC and WT littermates. Two biological replicates were performed for each experimental condition.

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Compensatory IKKa activation of classical NF-kB signaling during IKKb inhibition

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Transmission of Cricket paralysis virus via exosome-like vesicles during infection of Drosophila cells

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BRCA1-mimetic compound NSC35446.HCl inhits IKKB expression by reducing estrogen receptor alpha occupancy in the IKKB promoter and inhibts NF-κB activity in anti-estrogen resitant human breast cells

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