Transcriptomics

Dataset Information

0

MiR-34a controls proliferation and plasticity of early-progenitors in the normal mammary gland and in breast cancer


ABSTRACT: The miR-34 is a tumor-suppressor miRNA family involved in various human cancers, including breast. However, the role of such miRNAs in the control of mammary stem cells (MaSCs), early-progenitors and their tumor counterparts, (CSCs) lies largely unexplored. Here, we identified miR-34a as directly regulated by TP53 in primary mouse mammospheres. Expression of miR-34a is induced upon luminal commitment and differentiation and able to inhibit the expansion of the MaSCs/early-progenitor pool in a p53-independent fashion. Loss of function approaches revealed that miR-34 negatively controls both proliferation and fate commitment in mammary progenitors by modulating several pathways involved in epithelial cell plasticity and luminal-to-basal conversion. In particular, miR-34a negatively regulates Wnt/b-catenin signaling pathway, targeting multiple upstream regulators and, thus, modulating the expansion of the MaSCs/early-progenitor pool. These multiple roles of miR-34a were maintained in a model of human breast cancer, where chronic expression of miR-34a in triple-negative mesenchymal-like cells (enriched in CSCs) reduced tumor growth, restricted CSC pool and inhibited tumor propagation by promoting a luminal-like differentiation program.

ORGANISM(S): Mus musculus

PROVIDER: GSE99401 | GEO | 2018/06/26

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2023-08-21 | GSE227230 | GEO
2019-10-14 | PXD015278 | Pride
2016-11-17 | GSE75396 | GEO
2016-05-08 | GSE79050 | GEO
2022-06-14 | GSE205752 | GEO
2022-06-14 | GSE205754 | GEO
2019-12-21 | GSE142440 | GEO
2015-07-29 | E-GEOD-71423 | biostudies-arrayexpress
2017-03-08 | GSE89156 | GEO
2011-06-06 | E-GEOD-22143 | biostudies-arrayexpress