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RNA-Seq profiling of days 0, 7 and 18 kidney organoids differentiated from three separate vials of starting material

ABSTRACT: The kidney differentiation protocol takes induced pluripotent stem cells through to kidney organoid via directed differentiation in approximately 25 days. The cells are grown in a monolayer in a dish for seven days and are subjected to growth factors before being pelleted on day seven. The organoids then continue to differentiate as a 3D structure, with at least 8 distinct kidney cell types identifiable around day 18. This data has been generated to investigate the reproducibility of the kidney differentiation protocol using RNA sequencing from days 0, 7 and 18 organoids. Organoid differentiations were performed in parallel from three separate vials of starting material. The resulting RNA-seq data was analysed to identify genes with highly variable expression between vials.

ORGANISM(S): Homo sapiens

PROVIDER: GSE99469 | GEO | 2018/10/31

REPOSITORIES: GEO

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