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TrapSeq: An RNA Sequencing-based pipeline for the identification of genetrap insertions in mammalian cells

ABSTRACT: Current pipelines used to map genetrap insertion sites are based on inverse- or splinkerette-PCR methods, which despite their efficacy are prone to artifacts and do not provide information on the impact of the genetrap on the expression of the targeted gene. We developed a new method, which we named TrapSeq, for the mapping of genetrap insertions based on paired-end RNA sequencing. By recognizing chimeric mRNAs containing genetrap sequences spliced to an endogenous exon, our method identifies insertions that lead to productive trapping.

ORGANISM(S): Homo sapiens

PROVIDER: GSE99483 | GEO | 2018/02/14

REPOSITORIES: GEO

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