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Transcriptional profiling of WT and XBP1 Mutant Larvae

ABSTRACT: the purpose of the study is to compare the gene expression profile of WT vs. XBP1 Mutant Drosophila Larvae Methods: RNA sequencing was performed from 2nd instar Larvae of WT and XBP1 Mutant flies Results: Across the 4 samples, there was an average of 51 million mapped reads per sample with an average unique mapping rate of 83%. Using an adjusted p-value cutoff of 0.05, 154 genes were calculated to be differentially expressed. Conclusions: XBP1 affects gene expression profiling in drosophila larvae

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE99676 | GEO | 2017/06/05

SECONDARY ACCESSION(S): PRJNA389282

REPOSITORIES: GEO

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Drosophila melanogaster

Project description:Transcriptional profiling of WT and XBP1 Mutant Larvae

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Targeting the IRE1α/XBP1 endoplasmic reticulum stress response pathway in ARID1Amutant ovarian cancers

Project description:xenograft, patientderived xenograft and the genetic Arid1aflox/flox/Pik3caH1047R mouse models. Finally, B-I09 synergizes with inhibition of HDAC6, a known regulator of the ER stress response, in suppressing the growth of ARID1A-inactivated OCCCs. These studies reveal a promising therapeutic strategy for ARID1A-mutant OCCCs and define the IRE1?-XBP1 axis of the ER stress response as a targetable vulnerability for ARID1A-mutant OCCCs. Our findings indicate that pharmacological inhibition of the IRE1?-XBP1 pathway alone or in combination with HDAC6 inhibition represents an urgently needed therapeutic strategy for ARID1A-mutant ovarian cancers.

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Expression data in splenic DC subsets in wild type and Xbp1 deficient mice

Project description:Xbp1 is a major transcription factor in the unfolded protein response. To uncover its function in DCs we generated a conditional KO for Xbp1 in dendritic cells. We here compare the expression of mRNAs in two different splenic DC subpopulations, CD8a and CD11b DCs in both WT and KO mice. Reference: Inositol-requiring enzyme 1-alpha regulates CD8a dendritic cell function via regulated mRNA decay. Osorio et al, Nature Immunology (2014) Primary DC subsets were isolated and sorted from spleens from 3 different WT or CD11c-cre Xbp-1fl/fl mice. RNA was isolated, converted to cDNA and then hybridised on Affymetrix GeneChip Mouse Gene 1.0 ST Arrays (GPL6246).

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Expression data in splenic DC subsets in wild type and Xbp1 deficient mice

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Project description:Time course XBP1s ChIP-Seq in Xbp1 flx/flx (WT, wild-type) mice and AlbCre Xbp1 flx/flx (XBP1 LKO, liver-specific knockout) mice

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Time course Total RNA-Seq in Xbp1 flx/flx (WT, wild-type) mice and AlbCre Xbp1 flx/flx (XBP1 LKO, liver-specific knockout) mice

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