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Half-life determination for human B-cells (BL41)

ABSTRACT: RNA was labeled in BL41 cells by culturing cells for 60 min in media containing 100µM 4sU. Tc-RNA was separated into nt- and p-RNA. All three RNA subsets were subjected to microarray analysis. Only probe sets providing present calls in all RNA samples/subsets were included into the analysis We used microarrays to determine half-lives of mRNAs expressed in BL41 cells Keywords: Determination of mRNA half-life in human B-cells

ORGANISM(S): Homo sapiens

PROVIDER: GSE9973 | GEO | 2009/09/30

SECONDARY ACCESSION(S): PRJNA109051

REPOSITORIES: GEO

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Transcription profiling of human B-cells (BL41) for tc-, nt- and p-RNA half-life determination

Project description:RNA was labeled in BL41 cells by culturing cells for 60 min in media containing 100ÂµM 4sU. Tc-RNA was separated into nt- and p-RNA. All three RNA subsets were subjected to microarray analysis. Only probe sets providing present calls in all RNA samples/subsets were included into the analysis; We used microarrays to determine half-lives of mRNAs expressed in BL41 cells Experiment Overall Design: RNA was labeled in BL41 cells by culturing cells for 60 min in media containing 100ÂµM 4sU. Tc-RNA was separated into nt- and p-RNA. All three RNA subsets were subjected to microarray analysis.

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Expression data from NIH-3T3 cells used for half-life determination

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