Project description:Liver clock regulates transcription of hepatic genes in response to feeding. To explore the possibility that the microbiome influences this process, we measured the liver transcriptome in normal mice (Specific Pathogen Free or SPF mice) and compared it to the transcriptome in mice lacking microbiota (Germ Free or GF mice) at different time points over 24h. We used microarrays to detail the global programme of gene expression in liver of GF and SPF 10-12 weeks-old male C57Bl/6 male mice. There are 40 liver samples, each from an individual mouse. The samples are from germ free mice (GF) and specific pathogen free mice (SPF). Mice of both types were sacrificed at four time points: Zeitgeber Time 0, 6, 12, and 18. There are five replicates per condition.
Project description:The goal of this study is to understand the effect of microbiota on the cell frequencies and gene expression in skin. To achieve that we performed single cell sequencing of bladder cells from germ-free (GF) and specific pathogen free (SPF) mice
Project description:The goal of this study is to understand the effect of microbiota on the cell frequencies and gene expression in skin. To achieve that we performed single cell sequencing of lung cells from germ-free (GF) and specific pathogen free (SPF) mice
Project description:The goal of this study is to understand the effect of microbiota on the cell frequencies and gene expression in liver. To achieve that we performed single cell sequencing of liver cells from germ-free (GF) and specific pathogen free (SPF) mice
Project description:The goal of this study is to understand the effect of microbiota on the cell frequencies and gene expression in the colon. To achieve that we performed single cell sequencing of colon epithelium and lamina propria cells from germ-free (GF) and specific pathogen free (SPF) mice
Project description:To understand the effect of microbes on microRNAs in aorta, in this study, we examined expression of microRNA in the aorta of male (10 weeks old) germ-free mice and pathogen-free mice (control).
Project description:The goal of this study is to understand the effect of microbiota on the cell frequencies and gene expression in adipose tissue. To achieve that we performed single cell sequencing of epididymal fat pad cells from germ-free (GF) and specific pathogen free (SPF) mice
Project description:The goal of this study is to understand the effect of microbiota on the cell frequencies and gene expression in the small intestine . To achieve that we performed single cell sequencing of small intestine epithelium and lamina propria cells from germ-free (GF) and specific pathogen free (SPF) mice
Project description:Liver clock regulates transcription of hepatic genes in response to feeding. To explore the possibility that the microbiome influences this process, we measured the liver transcriptome in normal mice (Specific Pathogen Free or SPF mice) and compared it to the transcriptome in mice lacking microbiota (Germ Free or GF mice) at different time points over 24h. We used microarrays to detail the global programme of gene expression in liver of GF and SPF 10-12 weeks-old male C57Bl/6 male mice.