Metabolomics

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GNPS-Heart sections from Trypanosoma cruzi-infected mice


ABSTRACT: Mice were infected with Trypanosoma cruzi strains CL, X10/4 or X10/7. Hearts were collected 12 days, 3 months and 5 months post-infection from infected mice and uninfected age-matched controlled. Hearts were divided into four sections from the base of the heart to the apex. Metabolites were extracted by a two-step protocol, first with 50% methanol (aqueous extract) then with 3:1 dichloromethane:methanol (organic extract). Liquid chromatography separation was performed on a Kinetex C8 LC Column 50 x 2.1 mm, with water+0.1% formic acid and acetonitrile+0.1% formic acid as mobile phases.

INSTRUMENT(S): maXis

ORGANISM(S): Trypanosoma Cruzi (ncbitaxon:5693) Mus Musculus (ncbitaxon:10090)

SUBMITTER: Laura-Isobel McCall   James McKerrow  

PROVIDER: MSV000080450 | GNPS | Thu Jan 05 16:16:00 GMT 2017

REPOSITORIES: GNPS

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