Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

GNPS-Leishmania major ear infection Apr 2017

ABSTRACT: Mice were infected intradermally in the ear or left uninfected. Ear lesions, surrounding tissue and uninfected ear were collected 8 weeks post-infection. Metabolites were extracted by homogenization in 50% methanol (aqueous extract) followed by 3:1 dichloromethane:methanol (organic extract)

INSTRUMENT(S): impact

ORGANISM(S): Mus Musculus (ncbitaxon:10090) Leishmania Major (ncbitaxon:5664)

SUBMITTER: Laura-Isobel McCall

PROVIDER: MSV000081004 | GNPS | Tue Apr 25 16:59:00 BST 2017

REPOSITORIES: GNPS

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Differential analysis of Methylobacterium extorquens DM4 in methanol versus dichloromethane condition

Project description:Differential analysis of Methylobacterium extorquens DM4 in methanol versus dichloromethane condition using shotgun label free MS1 quantification approach

2018-04-25 | PXD006838 | Pride

SIK2-dependent Phosphoproteome and Interactome upon Salmonella infection

Project description:HeLa cells transfected with non-targeting control- and anti-SIK2 siRNA were left uninfected or infected with Salmonella and lysed 30 min pi. Following tryptic-digest peptides were labeled using TMT-pro 16-plex and phosphopeptides enriched. The endogenously tagged SIK2-HA IP workflow. Hela wild type or endogenous SIK2-HA-tagged HeLa cells were left uninfected or infected with Salmonella and lysed 1 h post-infection, followed by HA-IP, Tryptic digest, TMT-labeling and LC-MS/MS analysis.

2021-05-06 | PXD021859 | Pride

Proteogenomics of Hyphomicrobium sp. MC8b strain

Project description:This project is a proteomic comparison of Hyphomicrobium sp. MC8b grown with dichloromethane or with methanol. The datasets were obtained using the annotated genome of Hyphomicrobium sp. MC8b.

2021-09-09 | PXD021817 | Pride

GNPS - CeMiSt - Marine bryozoan Conopeum seruati extracts

Project description:Kupchan partitions of marine byrozoan Conopeum seruati, isolated from Jyllinge Harbor, Denmark. MBCe0238 - Aqueous extract MBCe0239 - n-butanol extract MBCe0240 - methanol extract MBCe0241 - dichloromethane extract

| MSV000090334 | GNPS

Proteomics of unsequenced Hyphomicrobium sp. MC8b strain explored by pan-proteomics

Project description:This project is a proteomic comparison of Hyphomicrobium sp. MC8b grown with dichloromethane or with methanol. The datasets were obtained using a pan-proteomic database built by merging the predicted proteomes from the annotated genomes of 13 Hyphomicrobium strains.

2021-09-09 | PXD021816 | Pride

Baylor-vaccine-mice-heart-tissue-positive-03-17-2021-all-6-organ

Project description:Female mice aged 6-7 weeks old were infected with the T. cruzi H1 strain. Mice were randomly divided into groups with 15 animals in each, with age matched naïve mice included as controls. Approximately 75 days post infection, mice were randomly divided into groups and treated with different treatment combinations: BNZ + Tc24 therapeutic vaccine + the STAT-3 inhibitor TTI-101, BNZ + Tc24 vaccine, and BNZ or TTI alone. Mice were humanely euthanized at 50, 75, 120 or 142 days post infection (DPI), and hearts samples were collected, divided into left atrium (LA), right atrium (RA), left ventricle top (LVT), left ventricle bottom (LVB), right ventricle top (RVT), right ventricle bottom (RVB). Heart tissue samples were extracted by 1:1 (v/v) methanol to water for aqueous phase and 3:1 (v/v) dichloromethane to methanol for organic phase. LC-MS/MS data acquisition was performed on a Q Exactive Plus Hybrid Quadrupole-Orbitrap Mass Spectrometer coupled to a Thermo Scientific Vanquish UHPLC with 1.7 um 100 Å Kinetex C8 column at 40°C. Mobile phase A was water with 0.1% (v/v) formic acid and mobile phase B was acetonitrile with 0.1% (v/v) formic acid.

2021-05-13 | MSV000087427 | MassIVE

Phosphoproteome upon Salmonella infection

Project description:HCT116 cells were grown in SILAC-DMEM (arginine and lysine-free medium), supplemented with dialyzed FCS (10%) and lysine [74µg/ml] and arginine [42µg/ml] as “light K0, R0”, “medium K4, R6” or “heavy K8, R10”. Cells were left uninfected or infected with Salmonella and lysed 30 min or 2h post-infection.

2021-05-06 | PXD023703 | Pride

Gene expression changes in liver upon LCMV Cl13 infection.

Project description:2-3 month old female C57Bl/6J mice were intravenously infected with 2x10^6 focus forming units of lymphocytic choriomeningitis virus strain Clone-13. Whole liver tissue was harvested in duplicated from uninfected control mice, uninfected 123d old mice, and from infected at 2, 8, 30, 60, and 123 days post infection. Three TMT 6-plex runs were carried out to monitor the changes in liver protein abundance during the entire course of infection. The first run included samples from day 2 and day 8 after infection along with the uninfected controls. Similarly, the second run included uninfected controls and samples from day 30 and day 60 after infection. The third run included samples day 123 after infection, the corresponding age matched control that was left uninfected for 123 days and the uninfected controls used in the other two runs. The uninfected controls were included in all the three runs as an internal control to monitor reproducibility between the runs.

2019-12-02 | PXD011122 | Pride

GNPS-Molecular changes associated with cutaneous leishmaniasis

Project description:Mice were infected subcutaneously with Leishmania major. Infected and uninfected footpads were collected 7 weeks post-infection. Metabolites were extracted by a two-step protocol, first with 50% methanol (aqueous extract) then with 3:1 dichloromethane:methanol (organic extract). Liquid chromatography separation was performed on a Kinetex 1.7 um C8 100 A, LC Column 50 x 2.1 mm, with water+0.1% formic acid and acetonitrile+0.1% formic acid as mobile phases.

2016-10-11 | MSV000080239 | GNPS

Organotypic human bronchial epithelial cultures (MucilAir) exposed to aqueous aerosol extracts of the 3R4F cigarette smoke and a novel tobacco product vapor

Project description:The current study was performed for analysis of the biological effects of vapor from novel tobacco vapor product in comparison with 3R4F cigarette smoke. This study was performed using a three-dimensional culture system composed of an air-liquid interface culture of primary normal human bronchial epithelial cells (MucilAir). The MucilAir tissues were subjected to 17 days of exposure to the aqueous extract of novel tobacco product vapor or 3R4F cigarette smoke. The number of differentially expressed genes increased in MucilAir tissues exposed to aqueous extract of each test product dependent on exposure duration. The number of differentially expressed genes was lower in the tissues exposed to aqueous extract of novel tobacco product vapor compared to the tissues exposed to aqueous extract of 3R4F cigarette smoke.

2020-04-15 | E-MTAB-8920 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data