Proteomics

Dataset Information

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Gene expression changes in liver upon LCMV Cl13 infection.


ABSTRACT: 2-3 month old female C57Bl/6J mice were intravenously infected with 2x10^6 focus forming units of lymphocytic choriomeningitis virus strain Clone-13. Whole liver tissue was harvested in duplicated from uninfected control mice, uninfected 123d old mice, and from infected at 2, 8, 30, 60, and 123 days post infection. Three TMT 6-plex runs were carried out to monitor the changes in liver protein abundance during the entire course of infection. The first run included samples from day 2 and day 8 after infection along with the uninfected controls. Similarly, the second run included uninfected controls and samples from day 30 and day 60 after infection. The third run included samples day 123 after infection, the corresponding age matched control that was left uninfected for 123 days and the uninfected controls used in the other two runs. The uninfected controls were included in all the three runs as an internal control to monitor reproducibility between the runs.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Liver

SUBMITTER: Alexandra Popa  

LAB HEAD: Dr. Andreas Bergthaler

PROVIDER: PXD011122 | Pride | 2019-12-02

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
M447-A01-O199-MM-P5186-1.raw Raw
M447-A02-O199-MM-P5186-1.raw Raw
M447-A03-O199-MM-P5186-1.raw Raw
M447-A04-O199-MM-P5186-1.raw Raw
M447-A05-O199-MM-P5186-1.raw Raw
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Publications


Infections induce complex host responses linked to antiviral defense, inflammation, and tissue damage and repair. We hypothesized that the liver, as a central metabolic hub, may orchestrate systemic metabolic changes during infection. We infected mice with chronic lymphocytic choriomeningitis virus (LCMV), performed RNA sequencing and proteomics of liver tissue, and integrated these data with serum metabolomics at different infection phases. Widespread reprogramming of liver metabolism occurred   ...[more]

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