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GNPS - Metabolomic profiles of 43 Euphorbia species

ABSTRACT: Extracts of forty-three Euphorbia species were collected from the greenhouses of the Living Collections of the Botanical Garden, Natural History Museum of Denmark, University of Copenhagen. Individual specimens of the same species were pooled, and the frozen plant material was disrupted with a pestle and mortar in liquid nitrogen and approximately 75 g was extracted with 975 ml ethyl acetate (VWR Chemicals, HiPerSolv Chromanorm) under sonication (Fisher Scientific) at 40 C during 2 h. The extracts were filtered and evaporated to dryness on a rotary evaporator. The dried extracts were then resuspended in acetonitrile (VWR Chemicals, HiPerSolv Chromanorm), extracted under sonication (Fisher Scientific) at 40 C during 15 min, filtered, and evaporated to dryness. Extracts were transferred to a 96-well plate (Falcon, 96-well plates, 0.34 ml, polypropylene) and dried with a vacuum centrifuge. Samples were redissolved in 3/7 vol/vol methanol (Fisher Scientific, HPLC Grade)/acetonitrile (Fisher Scientific, Optima LC/MS) to a concentration of 10 mg/ml, in a volume of 150 ul, with a 100 mM concentration of ammonium formate, sealed with Zone-Free Sealing Film (Excel Scientific), and centrifuged for 30 min at 2,000 r.p.m. at 4 C. MS analysis was performed on a qTOF Maxis II (Bruker Daltonics) mass spectrometer with an electrospray ionization (ESI) source, controlled by OTOF control and Hystar. For more details refer to the material and methods section in Ernst et al., 2019.

INSTRUMENT(S): maXis

ORGANISM(S): Eudicotyledons (ncbitaxon:71240)

SUBMITTER: Pieter Dorrestein

PROVIDER: MSV000081082 | GNPS | Mon May 15 04:13:00 BST 2017

REPOSITORIES: GNPS

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