Project description:This study focuses on the untargeted comparative metabolomics analysis of the endo-metabolome of both WT and acot-15 mutant C. elegans worms. Hydroxylamine was used to treat certain samples to capture the reactive electrophilic metabolites.
Project description:This study focuses on the untargeted comparative metabolomics analysis of the endo-metabolome of both WT and acot-15 mutant C. elegans worms. Hydroxylamine was used to treat certain samples to capture the reactive electrophilic metabolites.
Project description:Investigation of whole genome gene expression level changes in C. elegans eat-2; acs-22 and eat-2; pmk-1; acs-22 mutant compared to the eat-2 strain.
Project description:We used RNA-seq to identify gene expression changes in C. elegans pals-22 mutants, pals-22 pals-25 double mutants, and pals-25 mutants
Project description:This series of samples comprises multiple early embryonic time courses for C. elegans. Time courses consisting of 10 time points each for 4 different genotypes are included: wild-type (strain N2 grown on E. coli strain OP50), pie-1(zu154) (progeny of homozygous mutant mothers [Unc] of strain JJ532 grown on E. coli strain OP50), pie-1(zu154);pal-1(RNAi) (progeny of homozygous mutant mothers [Unc] of strain JJ532 grown on E. coli strain HT115 expressing pal-1 hairpin RNA), and mex-3(zu155);skn-1(RNAi) (progeny of homozygous mutant mothers [Dpy] of strain JJ518 grown on E. coli strain HT115 expressing skn-1 hairpin RNA). Embryos were manually staged by morphology at the 4-cell stage and allowed to develop in water for defined amounts of time at 22 degrees C. RNA was amplified as described (Baugh et al. Development, 2003; Baugh et al. Nucleic Acids Research, 2001). This series of samples comprises all replicate data reported by Baugh et al. (Development, 2005). Keywords: time-course