Project description:This study focuses on the untargeted comparative metabolomics analysis of the endo-metabolome of both WT and acot-15 mutant C. elegans worms. Hydroxylamine was used to treat certain samples to capture the reactive electrophilic metabolites.
Project description:This study focuses on the untargeted comparative metabolomics analysis of the endo-metabolome of both WT and acot-15 mutant C. elegans worms. Hydroxylamine was used to treat certain samples to capture the reactive electrophilic metabolites.
Project description:MDT-15/MED15 is a subunit of the Mediator complex and is known to regulate specific gene programs. We had previously profiled gene expression in C. elegans following mdt-15 depletion by RNAi. To complement this, we also performed gene expression profiling of the mdt-15(tm2182) hypomorhic mutant to identify MDT-15 regulated genes and developmental and/or physiological gene programs.
Project description:Purpose: To uncover immune genes and pathways that are modulated by the GPCR/NPR-15 Methods: RNA was extracted from synchronized L4 stage npr-15(tm12539) and WT animals grown at 20 C using Qiagen extraction kits and following standard methods Results: RNA seq analyses shows enriched and signficant upregulated immune, neuropeptide, synaptic signaling and metabolism genes and pathways that are dependent on NPR-15 Conclusions: Our study uncovered NPR-15 to be modulator of the innate immunity in C. elegans
Project description:Our study focuses in undersatnding the chromatin-associated function of the c. elegans IKB homologues nfki-1 and ikb-1. We this objective, we have generated different worm mutant strains and then analyzed by ChIP-seq possible changes in H3K36me3 and H3K27me3 marks in the WT , nfki-1, ikb-1 and double c elegans mutants.
Project description:Most aging hypotheses revolve around the accumulation of some sort of damage resulting in gradual physiological decline and ultimately death. Avoiding protein damage accumulation by enhanced turnover should slow down the aging process and extend lifespan. However, lowering translational efficiency extends rather than shortens lifespan in C. elegans. We studied turnover of individual proteins in the conserved Insulin/Insulin-like Growth Factor (IGF-1) receptor mutant daf-2 by combining Stable Isotope Labeling by Nitrogen-15 in Caenorhabditis elegans and LC-MS/MS. Intriguingly, the majority of proteins displayed prolonged half-lives in daf-2, while others remained unchanged, signifying that longevity is not supported by high protein turnover. This slow-down of protein turnover was most prominent for components of the translation machinery and mitochondria. In contrast, the high turnover of lysosomal hydrolases and very low turnover of cytoskeletal proteins remained largely unchanged in daf-2. The slow-down of protein dynamics and decreased abundance of the translational machinery may point at the importance of anabolic attenuation in lifespan extension as suggested by the hyperfunction theory.
Project description:Purpose: To uncover immune genes and pathways that are modulated by the GPCR/NPR-15 during S. aureus infection Methods: RNA was extracted from synchronized L4 stage npr-15(tm12539) and WT animals grown at 20 C and infected with S. aureus for 8 hours, followed by Qiagen extraction kits and following standard methods Results: RNA seq analyses shows enriched and signficant upregulated immune, neuropeptide, synaptic signaling and metabolism genes and pathways that are dependent on NPR-15 Conclusions: Our study uncovered NPR-15 to be modulator of the innate immunity in C. elegans under infection
Project description:We previously showed that the C. elegans Mediator subunit MDT-15 impacts expression of select genes involved in fatty acid (FA) metabolism (Taubert et al, Genes & Dev, 2006). To comprehensively identify processes downstream of MDT-15 in an unbiased manner, we set out to globally discover new MDT-15-dependent genes. Keywords: Expression profiling, RNAi depletion