Project description:Dataset 1 contains bacterial-fungal monocultures and tripartite co-cultures in which Scopulariopsis sp. JB370 are grown with 1) Hafnia alvei JB232 and 2) Pseudomonas psychrophila JB418 or Escherichia coli K12. Dataset 2 contains bacterial-fungal tripartite co-cultures in which Penicillium solitum #12 are grown with 1) Glutamicibacter arilaitensis JB182 or Brevibacterium linens JB5 and 2) Pseudomonas psychrophila JB418 or Escherichia coli K12. All cultures from each dataset were grown on 10% cheese curd agar (CCA) and extracted using acetonitrile. Extracts were analyzed via LC-MS(/MS) and processed with MZmine2 for analysis with MetaboAnalyst 5.0 and GNPS feature based molecular networking.
Project description:MS1 collected on qTOF, UPLC C18 gradient of 10-100% ACN over first 12 minutes, held at 100% ACN for minute 12-14. Fungi and Bacteria grown alone and pairwise on Cheese Curd Agar, naming is fungi first then bacterial partner (if it is a pairwise culture), then biological replicate number. CCA = cheese curd agar media control Ecoli = E. coli K12 JB418 = Pseudomonas psychrophila sp. JB418 12 = Penicillium solitum SAM = Penicillium camemberti RS17 = Penicillium atramentosum Fus = Fusarium domesticum JB370 = Scopulariopsis strain JB370 1655 = Scopulariopsis strain 165-5 Can = Candida catenulata Deb = Debaryomyces hansenii
Project description:MS2 collected on qTOF, UPLC C18 gradient of 10-100% ACN over first 12 minutes, held at 100% ACN for minute 12-14. Fungi and Bacteria grown alone and pairwise on Cheese Curd Agar, naming is fungi first then bacterial partner (if it is a pairwise culture), then biological replicate number.
CCA = cheese curd agar media control
Ecoli = E. coli K12
JB418 = Pseudomonas psychrophila sp. JB418
12 = Penicillium solitum
SAM = Penicillium camemberti
RS17 = Penicillium atramentosum
Fus = Fusarium domesticum
JB370 = Scopulariopsis strain JB370
1655 = Scopulariopsis strain 165-5
Can = Candida catenulata
Deb = Debaryomyces hansenii
Project description:Penicillium solitum grown on cheese curd agar with either E. coli and Pseudomonas psychrophila and extracted with acn. Data files processed with blank removal algorithm called BLANKA and compared to unprocessed files.
Project description:Bacterial-fungal monocultures and co-cultures in which Penicillium solitum #12 are grown with one of 4 bacterial growth partners (Glutamicibacter arilaitensis JB182, Brevibacterium linens JB5, Pseudomonas psychrophila JB418, Escherichia coli K12) grown on 10% cheese curd agar (CCA) and extracted using acetonitrile. Extracts were analyzed via LC-MS(/MS) and processed with MZmine2 for analysis with MetaboAnalyst 5.0 and GNPS feature based molecular networking. Dataset 3 contains monocultures and pairwise co-cultures in which full plate (100 mm) extractions were performed. Dataset 4 contains monocultures in which plug (30 mm) extractions were performed. Dataset 5 contains monocultures and pairwise co-cultures in that were grown regularly or on nitrocellulose membranes using plate count agar with milk and salt (PCAMS).
Project description:Penicillium camemberti grown on cheese curd agar with either E. coli or Pseudomonas psychrophila. Solid agar plates extracted with acn and run with RP UPLC C18 column on Bruker impact II qTOF.
Project description:MS2 collected on qTOF, UPLC C18 gradient of 10-100% ACN over first 12 minutes, held at 100% ACN for minute 12-14. Fungi grown alone on Cheese Curd Agar. CCA = cheese curd agar media control 12 = Penicillium solitum SAM = Penicillium camemberti RS17 = Penicillium atramentosum Fus = Fusarium domesticum JB370 = Scopulariopsis strain JB370 1655 = Scopulariopsis strain 165-5 Can = Candida catenulata Deb = Debaryomyces hansenii
Project description:Penicillium solitum grown on cheese curd agar with either E. coli and Pseudomonas psychrophila and extracted with acn. Data files unprocessed and compared to processed files with blank removal algorithm called BLANKA.
Project description:Penicillium solitum grown on cheese curd agar with either E. coli and Pseudomonas psychrophila and extracted with acn. Data files processed with blank removal algorithm called BLANKA and compared to unprocessed files.
Project description:Dataset 1 contains bacterial-fungal monocultures and tripartite co-cultures in which Scopulariopsis sp. JB370 are grown with 1) Hafnia alvei JB232 and 2) Pseudomonas psychrophila JB418 or Escherichia coli K12. Dataset 2 contains bacterial-fungal tripartite co-cultures in which Penicillium solitum #12 are grown with 1) Glutamicibacter arilaitensis JB182 or Brevibacterium linens JB5 and 2) Pseudomonas psychrophila JB418 or Escherichia coli K12. All cultures from each dataset were grown on 10% cheese curd agar (CCA) and extracted using acetonitrile. Extracts were analyzed via LC-MS(/MS) and processed with MZmine2 for analysis with MetaboAnalyst 5.0 and GNPS feature based molecular networking.