GNPS - MetFish: A Metabolomics Platform for Studying Microbial Communities in Chemically Extreme Environments

ABSTRACT: Here we report a facile method based on in situ chemical derivatization followed by extraction for analysis of metabolites and other chemicals in hypersaline samples, enabling for the first time direct LC-MS-based exo-metabolomics analysis in sample matrices containing up to 2 molar total dissolved salts. The method, MetFish, is applicable to molecules containing amine, carboxylic acid, carbonyl, or hydroxyl functional groups, and can be integrated into either targeted or untargeted analysis pipelines. MetFish was successfully applied in targeted and untargeted exo-metabolomics analyses of microbial consortia, quantifying amino acid dynamics in the exo-metabolome during community succession; in situ in a native prairie soil, whose exo-metabolome was isolated using a hypersaline extraction; and in input and produced fluids from a hydraulically fractured well, identifying dramatic changes in the exo-metabolome over time in the well.

INSTRUMENT(S): TSQ Quantum Ultra, Q Exactive Plus

ORGANISM(S): Environmental Samples <verrucomicrobiales> (ncbitaxon:48468)

SUBMITTER: Thomas Metz  

PROVIDER: MSV000085713 | GNPS | Fri Jul 10 10:47:00 BST 2020

REPOSITORIES: GNPS