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GNPS - Human NVU - Endothelial cells - Methamphetamine

ABSTRACT: For human brain microvascular endothelial cells, we used adult passage 3 cortical cells (hBMVECs, CellSystems, Kirkland, WA, USA). Cells were seeded at 13-15000 cells/cm2 in CSC complete medium (Cell Systems, Kirkland, WA, USA) on tissue culture plastic culture dishes coated with Attachment factor for 30 min (Cell Systems, Kirkland, WA, USA). Methamphetamine (Sigma-Aldrich, St Louis, MO, USA) was prepared at a stock concentration of 10mM in distilled water. Methamphetamine dilutions 50 micromolar were made in Endo and Neuro/Astro/Peri media respectively and applied to the cells for 26 hrs. Further info in "Proteomic and metabolomic characterization of human neurovascular unit cells in response to methamphetamine" Herland et al Adv Biosystems 2020

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Anna Herland

PROVIDER: MSV000085789 | GNPS | Mon Jul 20 15:21:00 BST 2020

REPOSITORIES: GNPS

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Human NVU - Endothelial cells - Methamphetamine

Project description:For human brain microvascular endothelial cells, we used adult passage 3 cortical cells (hBMVECs, CellSystems, Kirkland, WA, USA). Cells were seeded at 13-15000 cells/cm2 in CSC complete medium (Cell Systems, Kirkland, WA, USA) on tissue culture plastic culture dishes coated with Attachment factor for 30 min (Cell Systems, Kirkland, WA, USA). Methamphetamine (Sigma-Aldrich, St Louis, MO, USA) was prepared at a stock concentration of 10mM in distilled water. Methamphetamine dilutions 50 micromolar were made in Endo and Neuro/Astro/Peri media respectively and applied to the cells for 26 hrs. Further info in "Proteomic and metabolomic characterization of human neurovascular unit cells in response to methamphetamine" Herland et al Adv Biosystems 2020

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Project description:For human brain pericytes, we used fetal cells passage 3 (ScienCell, San Diego, CA, USA ) seeded Pericyte medium (ScienCell, San Diego, CA, USA) at density 8-15000 cells/cm2 tissue culture plastic culture dishes coated with Attachment Factor for 30 min (Cell Systems, Kirkland, WA, USA). Methamphetamine (Sigma-Aldrich, St Louis, MO, USA) was prepared at a stock concentration of 10mM in distilled water. Methamphetamine dilutions 50 micromolar were made in Endo and Neuro/Astro/Peri media respectively and applied to the cells for 26 hrs. Further info in "Proteomic and metabolomic characterization of human neurovascular unit cells in response to methamphetamine" Herland et al Adv Biosystems 2020

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