Project description:These experiments were designed to determine the usefulness of the spurge/cassava euphorbiaceae arrays for investigating gene expression in other euphorbs. Keywords: heterologous hybridization euphorbia
Project description:These experiments were designed to determine the usefulness of the spurge/cassava euphorbiaceae arrays for investigating gene expression in other euphorbs. two color dye swap design
Project description:The leaf transcriptome of the nickel hyperaccumulator Leucocroton havanensis (Euphorbiaceae) living on serpentine Cuabal, from Cuba, was compared to the closely related non-accumulator Lasiocroton microphyllus living on Gallery forest on limestone soil, to identity differentially expressed genes potentially involved in Ni hyperaccumulation.
Project description:Mycoplasma gallisepticum (MG) is one of the smallest free-living and self-replicating organisms, it is characterized by lack of cell wall and reduced genome size. As a result of genome reduction, MG has a limited variety of DNA-binding proteins (DBP) and transcription factors. To investigate the dynamic changes of the proteomic profile of MG nucleoid, that may assist in revealing its mechanisms of functioning, regulation of chromosome organization and stress adaptation, a quantitative proteomic study was performed on MG nucleoids obtained from the cell culture in logarithmic and stationary phases of synchronous growth. MG cells were grown in a liquid medium with a 9h starvation period. Nucleoids were obtained from the cell culture at the 26th and the 50th hour (logarithmic and stationary phases respectively) by sucrose density gradient centrifugation. LC-MS analysis was carried out on an Ultimate 3000 RSLCnano HPLC system connected to a Fusion Lumos mass spectrometer, controlled by XCalibur software (ThermoFisher Scientific) via a nanoelectrospray source (Thermo Fisher Scientific). For comprehensive peptide library generation one sample from each biological replicate was run in DDA mode. Then, all the sample were run in a single LC-MS DIA run. Identification of DDA files and DIA quantitation was performed with MaxQuant and Skyline software.
Project description:This study investigated the ability of two novel adjuvant formulations, QCDC (Quil A/cholesterol/DDA/Carbopol) and QCDCR (QCDC/Bay R1005), in combination with a recombinant profilin vaccine, to modulate host protective immunity and to alter new gene expression during experimental avian coccidiosis.