GNPS Quantification and validation (MRM) of analysis - compounds isolated from Heteropterys umbellata (Malpighiaceae)

ABSTRACT: Analyses of Heteropterys umbellata (Malpighiaceae) stems and leaves extracts. Plants were collected in two different Brazilian States (Sao Paulo and Minas Gerais). Dried plant material was extracted with EtOH80%. Positive ionization mode analyses. LC-MS/MS analyses were performed in a Shimadzu HPLC using a Phenomenex C18-Luna (250 mm x 4.6 mm, 5.0 um) column and an Amazon SL (Bruker Daltonics) mass spectrometer (ion trap) equipped with an ESI source. Multiple reaction monitoring (MRM) mode for quantification of isolated compounds. The transitions 499.10->319.10, 501.10->365.10, 602.20->466.10, and 296.10->278.00 were chosen to monitor 3,5-di-O-caffeoylquinic acid (isochlorogenic acid A); 1,2,6-tris-O-[3-nitropropanoyl]-beta-glucopyranose (karakin); 1,2,3,6-tetrakis-O-[3-nitropropanoyl]-beta-glucopyranose; and sodium diclofenac (used as internal standard), respectively.

INSTRUMENT(S): Amazon SL (Bruker Daltonics amaZon series)

ORGANISM(S): Heteropterys Umbellata

SUBMITTER: Vanderlan da Silva Bolzani  

PROVIDER: MSV000089350 | GNPS | Sat Apr 30 16:48:00 BST 2022

REPOSITORIES: GNPS