GNPS California Current Ecosystem P2107 Research Cruise
Ontology highlight
ABSTRACT: DDA non-targeted LC-MS/MS, PPL-SPE extracted marine organic matter. Positive Mode. Samples taken during the P2107 CCE LTER Research Cruise onboard the R/V Revelle.
Project description:DDA non-targeted LC-MS/MS, PPL-SPE extracted marine organic matter. Positive Mode. Samples taken from during the Scripps Pier Diel Project 2022.
Project description:DDA non-targeted LC-MS/MS, PPL-SPE extracted marine organic matter. Positive and Negative Mode. Samples taken during Mo'orea April 2022 fieldwork.
Project description:DDA non-targeted LC-MS/MS, PPL-SPE extracted marine organic matter. Positive Mode. Samples taken from during the Scripps Pier Diel Project 2022.
Project description:Interlab Study of LC-MS/MS analyis of Marine Dissolved Organic Matter from SIO Pietr (San Diego, California, USA) and algae extracts, extracted via PPL SPE
Project description:Dissolved organic matter prepared via PPL SPE from cultures of Synechococcus WH7803. Biological triplicate samples were prepared of DOM released via exudation, mechanical lysis, and viral lysis by phage S-SM1.
Project description:Small, biologically produced, organic molecules called metabolites play key roles in microbial systems where they directly mediate exchanges of nutrients, energy, and information. However, the study of dissolved polar metabolites in seawater and other environmental matrices has been hampered by analytical challenges including high inorganic ion concentrations, low analyte concentrations, and high chemical diversity. Here we show that a cation-exchange solid phase extraction (CX-SPE) sample preparation approach separates positively charged and zwitterionic metabolites from seawater and freshwater samples, allowing their analysis by liquid chromatography-mass spectrometry (LC-MS). We successfully extracted 69 known compounds from an in-house compound collection and evaluated the performance of the method by establishing extraction efficiencies and limits of detection (pM to low nM range) for these compounds. CX-SPE extracted a range of compounds including amino acids and compatible solutes, resulted in very low matrix effects, and performed robustly across large variations in salinity and dissolved organic matter (DOM) concentration. We compared CX-SPE to an established solid phase extraction procedure (PPL-SPE) and demonstrate that these two methods extract fundamentally different fractions of the dissolved metabolite pool with CX-SPE extracting compounds that are on average smaller and more polar. We use CX-SPE to analyze four environmental samples from distinct aquatic biomes, producing some of the first CX-SPE dissolved metabolomes. Quantified compounds ranged in concentration from 0.0093 nM to 49 nM and were composed primarily of amino acids (0.15 – 16 nM) and compatible solutes such as TMAO (0.89 – 49 nM) and glycine betaine (2.8 – 5.2 nM).
Project description:Optimization of Solid Phase Extraction Columns (C18, HBL, PPL) for non-targeted LC-MS/MS analysis of river dissolved organic matter.