Dataset Information

GNPS - Native MS of Daptomycin in Nanodiscs

ABSTRACT: Native MS data examining incorporation of daptomycin into intact nanodiscs at different concentrations and with different lipids

INSTRUMENT(S): Q Exactive UHMR

ORGANISM(S): Bacteria

SUBMITTER: Michael Marty

PROVIDER: MSV000090729 | GNPS | Wed Nov 16 15:21:00 GMT 2022

REPOSITORIES: GNPS

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Publications

Investigating Daptomycin-Membrane Interactions Using Native MS and Fast Photochemical Oxidation of Peptides in Nanodiscs.

Reid Deseree J DJ Dash Tapasyatanu T Wang Zhihan Z Aspinwall Craig A CA Marty Michael T MT

Analytical chemistry 20230308 11

Daptomycin is a cyclic lipopeptide antibiotic that targets the lipid membrane of Gram-positive bacteria. Membrane fluidity and charge can affect daptomycin activity, but its mechanisms are poorly understood because it is challenging to study daptomycin interactions within lipid bilayers. Here, we combined native mass spectrometry (MS) and fast photochemical oxidation of peptides (FPOP) to study daptomycin-membrane interactions with different lipid bilayer nanodiscs. Native MS suggests that dapto ...[more]

PMID: 36888920

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