Dataset Information

FPOP of Daptomycin in Nanodiscs

ABSTRACT: FPOP data examining solvent exposure of daptomycin at different concentrations in the presence of nanodiscs with different lipids

INSTRUMENT(S): Synapt XS

ORGANISM(S): Bacteria (ncbitaxon:2)

SUBMITTER: Michael Marty

PROVIDER: MSV000090629 | MassIVE | Tue Nov 01 16:43:00 GMT 2022

REPOSITORIES: MassIVE

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Publications

Investigating Daptomycin-Membrane Interactions Using Native MS and Fast Photochemical Oxidation of Peptides in Nanodiscs.

Reid Deseree J DJ Dash Tapasyatanu T Wang Zhihan Z Aspinwall Craig A CA Marty Michael T MT

Analytical chemistry 20230308 11

Daptomycin is a cyclic lipopeptide antibiotic that targets the lipid membrane of Gram-positive bacteria. Membrane fluidity and charge can affect daptomycin activity, but its mechanisms are poorly understood because it is challenging to study daptomycin interactions within lipid bilayers. Here, we combined native mass spectrometry (MS) and fast photochemical oxidation of peptides (FPOP) to study daptomycin-membrane interactions with different lipid bilayer nanodiscs. Native MS suggests that dapto ...[more]

PMID: 36888920

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