GNPS - Effect of rumen-protected methionine on metabolic profile in liver, muscle and blood serum samples of growing German Simmental bulls at restricted protein supply

ABSTRACT: This study aimed to determine the metabolic response of growing Fleckvieh bulls fed diets low in crude protein (CP) supplemented with rumen-protected methionine (RPM). In total, 69 bulls were assigned to three dietary treatments (n=23/group): Positive control (CON; 13.7% CP; 2.11 g methionine/kg DM) and negative control deficient in crude protein (RED; 9.04% CP; 1.56 g methionine/kg DM) with supplemented RPM (RED+MET; 9.04% CP; 2.54 g methionine/kg DM). At slaughter, samples of liver, muscle and blood serum were taken and underwent subsequent metabolomics profiling using a UHPLC-QTOF-MS system. A total of 6,540 features could be detected. 70 metabolites in the liver, six metabolites in muscle and 60 metabolites in blood serum were affected (p < 0.05) due to die-tary treatments. In total, five metabolites could be reliably annotated and were thus subject to subsequent univariate analysis. Reduction in dietary CP had hardly any effect on metabolite abundance in target tissues of both RED and RED+MET bulls as compared CON bulls. Addition of RPM altered hepatic anti-oxidant status in RED+MET bulls as compared to both RED and CON bulls (cysteine glutathione disulfide) . Results exemplify nutrient partitioning in growing Fleckvieh bulls: bulls set maintenance as prevailing metabolic priority (homeostasis) and as a second priority, nutrient trafficking is directed towards special metabolic functions, such as an-ti-oxidant pathways.

INSTRUMENT(S): TripleTOF 6600

ORGANISM(S): Bos Taurus (ncbitaxon:9913)

SUBMITTER: Thomas Ettle  

PROVIDER: MSV000092367 | GNPS | Fri Jul 07 03:22:00 BST 2023

REPOSITORIES: GNPS