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GNPS - Octocoral_briarane_FBMN_A widespread natural product gene cluster family to briarane diterpenoids in coral animals

ABSTRACT: Soft corals are unique amongst animals in their prolific production of bioactive terpenoid natural products that rival the chemical diversity of plants and microbes. We recently established that octocorals uniformly express terpene cyclases and that their encoding genes often reside within putative biosynthetic gene clusters, a feature uncommon in animal genomes. In this work, we report the discovery and characterization of a widespread gene cluster family for the biosynthesis of briarane diterpenoids that number over 600 molecules distinct to corals. We sequenced five genomes from evolutionarily discrete families of briarane-producing octocorals, including the chromosomally resolved precious coral Corallium rubrum, and identified a common five-gene cluster composed of a terpene cyclase, three cytochrome P450s, and a short-chain dehydrogenase. Using Escherichia coli and Saccharomyces cerevisiae as hosts and homologous briarane biosynthesis genes from seven corals, we reconstituted the biosynthesis of cembrene B g-lactone, which contains the g-lactone structural feature distinctive of briarane diterpenoids. The discovery of the genomic basis of briarane biosynthesis not only allows for its biological examination across coral species but establishes that animals, like microbes and plants, also employ gene cluster families to produce specialized metabolites.

INSTRUMENT(S): 6530A Q-TOF LC/MS

ORGANISM(S): Corallium Rubrum (ncbitaxon:142104) Dichotella Gemmacea (ncbitaxon:767270) Renilla Koellikeri (ncbitaxon:6135) Stylatula Elongata (ncbitaxon:1214922) Briareum Asbestinum (ncbitaxon:86543) Erythropodium Caribaeorum (ncbitaxon:86550)

SUBMITTER: Bradley Moore

PROVIDER: MSV000094792 | GNPS | Fri May 17 15:50:00 BST 2024

REPOSITORIES: GNPS

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GNPS - Octocoral_briarane_FBMN_A widespread natural product gene cluster family to briarane diterpenoids in coral animals

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