Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

GNPS - BRA006 (Micromonospora sp.), BRA010 (Streptomyces sp.) and BRA177 (Actinomadura sp.) metabolome obtained in LC-MS/MS in DDA mode

ABSTRACT: Data acquired by LC-MS/MS DDA mode of methanolic extracts (methanol:water) for actinomycetes BRA006 (Micromonospora sp.), BRA010 (Streptomyces sp.) and BRA177 (Actinomadura sp.)

INSTRUMENT(S): Impact II QToF Bruker Daltonics

ORGANISM(S): Streptomyces Sp. (ncbitaxon:1931) Micromonospora Sp. (ncbitaxon:1876) Actinomadura Sp. (ncbitaxon:1989)

SUBMITTER: Ricardo Roberto da Silva

PROVIDER: MSV000095044 | GNPS | Fri Jun 14 12:47:00 BST 2024

REPOSITORIES: GNPS

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

BRA006 (Micromonospora sp.), BRA010 (Streptomyces sp.) and BRA177 (Actinomadura sp.) metabolome obtained in LC-MS/MS with a scheduled precursor list (SPL)

Project description:Data obtained by LC-MS/MS of methanolic extracts (methanol:water) for the actinomycetes BRA006 (Micromonospora sp.), BRA010 (Streptomyces sp.) and BRA177 (Actinomadura sp.), where the precursor ions were guided by a scheduled precursor list (SPL).

2024-06-24 | MSV000095129 | MassIVE

BRA006 (Micromonospora sp.), BRA010 (Streptomyces sp.) and BRA177 (Actinomadura sp.) metabolome obtained in Full Scan

Project description:Data acquired by LC-MS in full scan mode (MS1) in a five point dilution series of methanolic extracts (methanol:water) for actinomycetes BRA006 (Micromonospora sp.), BRA010 (Streptomyces sp.) and BRA177 (Actinomadura sp.).

2024-06-14 | MSV000095042 | MassIVE

Total protein of S. aureus treated and untreated with Streptomyces sp. GKU 223

Project description:This study is aimed to isolate marine actinomycetes from sediments from Andaman and the Gulf of Thailand. All 101 marine actinomycetes were screened for anti-biofilm activity. Streptomyces sp. GKU223 showed significantly inhibited biofilm formation of S. aureus. The evaluation of supernatants of anti-biofilm activity produced by Streptomyces sp. GKU223 has been performed. Since the interaction between marine actinomycetes and biofilm forming bacteria has never been investigated, proteomic analysis has been used to identify whole cell proteins involved in anti–biofilm activity. Understanding the interaction at molecular level will lead to sustainably use for anti-biofilm producing marine actinomycetes in pharmaceutical and medicinal applications in the future.

2018-08-20 | PXD010815 | JPOST Repository

Analysis of Escherichia coli treated and untreated with Streptomyces sp. GKU 257-1

Project description:This study is aimed to isolate marine actinomycetes from sediments from Andaman and the Gulf of Thailand. All 101 marine actinomycetes were screened for anti-biofilm activity. Streptomyces sp. GKU 257-1 showed significantly inhibited biofilm formation of E. coli. The evaluation of supernatants of anti-biofilm activity produced by Streptomyces sp. GKU 257-1 has been performed. Since the interaction between marine actinomycetes and biofilm forming bacteria has never been investigated, proteomic analysis has been used to identify whole cell proteins involved in anti–biofilm activity. Understanding the interaction at molecular level will lead to sustainably use for anti-biofilm producing marine actinomycetes in pharmaceutical and medicinal applications in the future.

2019-09-07 | PXD011027 | JPOST Repository

Project description:WTCCC2 project Schizophrenia (SP) samples

| EGAD00010000262 | EGA

Co-adjuvanting DDA/TDB liposomes with a TLR7 agonist allows for IgG2a/c class-switching in the absence of Th1 cells

Project description:Class-switching to IgG2a/c in mice is a hallmark response to intracellular pathogens. T cells can promote class-switching and the predominant pathway for induction of IgG2a/c antibody responses has been suggested to be via stimulation from Th1 cells. We previously formulated CAF®01 (cationic liposomes containing dimethyldioctadecylammonium bromide (DDA) and Trehalose-6,6-dibehenate (TDB)) with the lipidated TLR7/8 agonist 3M-052 (DDA/TDB/3M-052), which promoted robust Th1 immunity in newborn mice. When testing this adjuvant in adult mice using the recombinant Chlamydia trachomatis (C.t.) vaccine antigen CTH522, it similarly enhanced IgG2a/c responses compared to DDA/TDB, but surprisingly reduced the magnitude of the IFN-g+ Th1 response in a TLR7 agonist dose-dependent manner. Single cell RNA-sequencing revealed that DDA/TDB/3M-052 liposomes initiated early transcription of class-switch regulating genes directly in pre-germinal center B cells. Mixed bone marrow chimeras further demonstrated that this adjuvant did not require Th1 cells for IgG2a/c switching, but rather facilitated TLR7-dependent T-bet programming directly in B cells. This study underlines that adjuvant-directed IgG2a/c class-switching in vivo can occur in the absence of T cell help, via direct activation of TLR7 on B cells and positions DDA/TDB/3M-052 as a powerful adjuvant capable of eliciting type I-like immunity in B cells without strong induction of Th1 responses.

2023-11-17 | GSE245073 | GEO

Streptomyces sp. M7 LC-MS - Streptomyces sp. M7 has demonstrated ability to remove lindane from culture media and soils

Project description:Streptomyces sp. M7 has demonstrated ability to remove lindane from culture media and soils. In this study, we used MS-based label-free quantitative proteomic to understand lindane degradation and its metabolic context in Streptomyces sp. M7. We identified the proteins involved in the up-stream degradation pathway. Our results demonstrated that mineralization of lindane is feasible since proteins from an unusual down-stream degradation pathway were also identified. Degradative steps were supported by an active catabolism that supplied energy and reducing equivalents in the form of NADPH. This is the first study in which degradation steps of an organochlorine compound and metabolic context are elucidate in a biotechnological genus as Streptomyces. These results serve as basement to study other degradative actinobacteria and to improve the degradation processes of Streptomyces sp. M7.

2018-08-10 | PXD010607 | Pride

Proteomics analysis of streptomyces sp. MBT76 demonstrates the production of qinichelins, new mixed catecholate-hydroxamate siderophores

Project description:Actinomycete genomes contain a plethora of orphan gene clusters encoding unknown secondary metabolites, and representing a huge unexploited pool of chemical diversity. The explosive increase in genome sequencing and the massive advance of bioinformatic tools have revolutionized the rationale for natural product discovery from actinomycetes. In this context, we applied a genome mining approach to discover a group of unique catecholate-hydroxamate siderophores termed as qinichelins from Streptomyces sp. MBT76. Quantitative proteomics statistically correlated a gene cluster of interest (qch) to its unknown chemotype (qinichelin), after which structural elucidation of isolated qinichelin was assisted by bioinformatics analysis and verified by MS2 and NMR experiments. Strikingly, intertwined functional crosstalk among four separately located gene clusters was implicated in the biosynthesis of qinichelins.

2018-10-24 | PXD006577 | Pride

GC-MS extract Streptomyces sp. RC4

Project description:Title: Effects of Different Media and Solvents on Biological Activities and Secondary Metabolites Profiles of Coral-Derived Streptomyces sp. RC4 Authors: RIZKY, RISA NOFIANI, RUDIYANSYAH, CANTIKA DYLANI PUTRI, DES SAPUTRO WIBOWO, FITRI SETYONINGRUM

2024-10-01 | MSV000095999 | MassIVE

Examination of polyester-polyurethane degradation by Streptomyces sp. PU10

Project description:This study aimed to investigate the variations in the protein composition of Streptomyces sp. PU10 when cultivated with either Impranil (polyestere-polyurethane) or glucose as the carbon source. We analyzed both the intracellular and extracellular protein fractions to gain insights into the intricate processes involving PU degradation, intermediate metabolic pathways in PU degradation, and the connection between primary and secondary metabolism within Streptomyces sp. PU10.

2024-03-29 | PXD047534 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data