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GNPS - Multi-ROI, multi-condition, and multi-replicate metabolite MALDI imaging of mouse heart and pancreas

ABSTRACT: Mouse heart and pancreas tissues for imaging mass spectrometry were removed from animal organs, frozen on dry ice, and then stored at -80-degree C until analysis. 12-micrometer mouse heart tissues were sectioned using a Leica CM 3050S Research Cryostat (Leica Biosystems, Wetzlar, Germany) using -25-degree C chamber temperature and -23-degree C object temperature, prior to thaw mounting onto indium tin oxide-coated microscope slides (Delta Technologies, Loveland, CO, USA). A 10 mg/mL solution of 1,5-diaminonaphthalene (DAN) MALDI matrix layer was applied to the microscope slide using an HTX M5 TM Sprayer (HTX Technologies, LLC, Chapel Hill, NC, USA) with 0.1 mL/min flow rate over 4, 6, or 8 passes. MALDI imaging mass spectrometry was performed on a 7T solariX Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometer equipped with a dynamically harmonized ParaCell XR (Bruker Daltonics, Bremen, Germany). Analysis was performed in negative ion mode from m/z 75 to 500 with a 1 megaword transient (~0.4 s ICR transient length). A 117 (+/-) 75 m/z continuous accumulation of selected ions (CASI) window was used to perform gas-phase enrichment of low m/z metabolites. The MALDI source is equipped with a Smartbeam II Nd:YAG MALDI laser and was used to sample at a pixel spacing of 200 micrometers in the x and y dimensions using 200 laser shots per pixel (minimum laser focus, 2 kHz frequency) with Smart Walk enabled

INSTRUMENT(S): 7T solariX FT-ICR

ORGANISM(S): Mus Musculus

SUBMITTER: Boone M. Prentice

PROVIDER: MSV000095123 | GNPS | Sun Jun 23 17:50:00 BST 2024

REPOSITORIES: GNPS

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Multi-ROI, multi-condition, and multi-replicate metabolite MALDI imaging of mouse heart and pancreas

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