Proteomics

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Data in support of Enhanced amyloid beta proteoform detection in an Alzheimer’s disease model by integrated matrix composition enhanced MALDI Imaging and laser capture microdissection

ABSTRACT: This study has investigated several parameters to obtain increased detection of the amyloid plaque intact protein profile, including a comparison of commonly used MALDI matrices, acid based reduction of signal suppression as well as the combination of strong acid based protein aggregate extraction from laser microdissection plaques to increase detection of proteoforms. Female APPPS1-21 transgenic mice were analyzed by MALDI Imaging with different matrices on a Ultraflextreme MALDI TOF/TOF instrument followed by data analysis in SCiLS Lab software. A combination of formic acid treatment of laser capture microdissected plaques was further utilized to expand the analysis and validation of amyloid plaques.

INSTRUMENT(S): ultraflex

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

DISEASE(S): Alzheimer's Disease

SUBMITTER: Allan Stensballe  

LAB HEAD: Allan Stensballe

PROVIDER: PXD009353 | Pride | 2021-03-25

REPOSITORIES: Pride

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Dataset's files

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Action DRS
Tg1_CHCA_02TFA.zip Other
Tg1_DHB_02PA.zip Other
Tg1_DHB_02TFA.zip Other
Tg1_SA_02TFA.zip Other
Tg3_CHCA_02TFA.zip Other
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Publications

Dual strategy for reduced signal-suppression effects in matrix-assisted laser desorption/ionization mass spectrometry imaging.

Bastrup Joakim J   Birkelund Svend S   Asuni Ayodeji A AA   Volbracht Christiane C   Stensballe Allan A  

Rapid communications in mass spectrometry : RCM 20191101 22

<h4>Rationale</h4>The molecular complexity of tissue features several signal-suppression effects which reduce the ionization of analytes significantly and thereby weakens the quality of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) imaging (MALDI imaging). We report a novel approach in MALDI imaging by reducing signal-suppression effects for the analysis of beta-amyloid (Aβ) plaques, one pathological hallmark of Alzheimer's disease (AD).<h4>Methods</h4>We analyzed Aβ  ...[more]

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