Metabolomics

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GNPS - Quantification N-acyl lipids (stool samples and microbial monocultures)


ABSTRACT: Stool samples are part of the MSV000092833 dataset (HNRC cohort). Microbial monocultures contained histamine, cadaverine, and putrescine added to the media. MS/MS data was acquired on a Q Exactive Orbitrap in positive ionization mode. Quantification of N-acyl lipids was performed (histamine-C2:0, histamine-C3:0, histamine-C4:0, histamine-C5:0, cadaverine-C2:0, cadaverine-C3:0, cadaverine-C5:0, cadaverine-C6:0, and dopamine-C2:0), while retention time matching was also done for histamine-C6:0, cadaverine-C7:0, serotonin-C2:0, and tryptophan-C3:0. All the N-acyl lipids used were acquired as pure standards from commercial vendors.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Prevotella Buccae D17 (ncbitaxon:575611) Roseburia Inulinivorans Dsm 16841 (ncbitaxon:622312) Dorea Longicatena Dsm 13814 (ncbitaxon:411462) Holdemanella Biformis (ncbitaxon:1735) Streptococcus Thermophilus Lmd-9 (ncbitaxon:322159) Megasphaera Sp. (ncbitaxon:2023260) Catenibacterium Mitsuokai Dsm 15897 (ncbitaxon:451640) Eubacterium Siraeum Dsm 15702 (ncbitaxon:428128) Homo Sapiens (ncbitaxon:9606) Collinsella Aerofaciens Atcc 25986 (ncbitaxon:411903) Desulfovibrio Piger Atcc 29098 (ncbitaxon:411464)

SUBMITTER: Pieter Dorrestein  

PROVIDER: MSV000096049 | GNPS | Tue Oct 08 16:18:00 BST 2024

REPOSITORIES: GNPS

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