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GNPS - Dietary isoleucine content defines the metabolic and molecular response to a Western diet

ABSTRACT: Untargeted lipidomics of liver samples from female and male DBA/2J or C57BL/6J mice fed a control diet, Western diet, or high- or low-isoleucine Western diet. Both positive and negative mode are included.

INSTRUMENT(S): 6545 Q-TOF LC/MS

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Judith Simcox

PROVIDER: MSV000096669 | GNPS | Mon Dec 16 08:44:00 GMT 2024

REPOSITORIES: GNPS

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Dietary isoleucine content defines the metabolic and molecular response to a Western diet

Project description:Untargeted lipidomics of liver samples from female and male DBA/2J or C57BL/6J mice fed a control diet, Western diet, or high- or low-isoleucine Western diet. Both positive and negative mode are included.

2024-12-16 | MSV000096669 | MassIVE

Effect of depletion of TAS1R3 on testicular gene expression in mice fed long-term western diet

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Dietary fiber deficiency promotes cognitive impairment through the gut-hippocampal axis

Project description:C57BL/6J male mice (11 weeks old) were randomly divided into two groups fed either the control (Con) diet (LabDiet 5010) or fiber deficiency (FD) diet for 15 weeks.

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The effects of a low isoleucine diet on the liver expression of aged C57BL/6J.Nia mice of both sexes

Project description:Dietary isoleucine is a significant determinant of metabolic health and longevity. In this study, we found that even at an advanced age, both male and female mice received significant metabolic benefits from a low isoleucine diet. We collected liver tissues for transcriptomic analysis and the results found that the majority of age-driven changes is ameliorated with the administration of low isoleucine diet.

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Project description:A soy diet worsens the progression of an inherited form of hypertrophic cardiomyopathy (HCM) in male mice when compared to casein-fed mice. Females are largely resistant to this diet effect and better preserve cardiac function. We hypothesized that the abundant phytoestrogens found in soy are mainly responsible for this diet-dependent phenotype. Indeed, feeding male mice a phytoestrogen-supplemented casein-based diet can recapitulate the negative outcome seen when male mice are fed a standard soy-based diet. To gain mechanistic insights into disease pathogenesis, we used Affymetrix microarrays to profile gene expression in left ventricular tissue from 2 month old HCM male and female mice as well as wild-type littermate controls. These mice were fed a phytoestrogen (genistein + daidzein)-supplemented casein-based diet. We identified a number of molecular pathways that could explain both the male and female phenotypes. Hearts from male and female wild-type or HCM C57BL/6 mice fed a phytoestrogen-supplemented (genistein and daidzein mix) casein-based diet were excised at 2 months of age. Total RNA was extracted from left ventricles. Biotin-labeled amplified RNA was hybridized to Affymetrix Mouse Genome 430 2.0 microarrays.

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Expression profiling of liver tissue from (C57BL/6J X C3H/HeJ)F2 mice on ApoE null backgrounds

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