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Omics score: 1009

Gel-based mass spectrometric analysis of hippocampal transmembrane proteins using high resolution LTQ Orbitrap Velos Pro.

ABSTRACT: Data from ProteomeXchange, PXD ID: PXD000311. Enzyme: trypsin, file: F017572.dat-ACA-SDS_T3.mzml. Published as part of Proteomics. 2014 Jul 10 . From the Abstract: {{i}} ... Proteins were extracted from pooled whole mouse brains, enriched membrane fractions were prepared using either two commercially available kits or 6-aminocaproic acid under denaturing or native conditions followed by gel-based proteomic approaches using blue native (BN-) and SDS-PAGE with subsequent in-gel digestion with several proteases, chymotrypsin, trypsin followed by nano-LC-ESI-MS/MS analysis on LTQ Orbitrap Velos Pro ... {{/i}}

INSTRUMENT(S): Instrument

ORGANISM(S): Mus_musculus_viruses, Mouse

DISEASE(S): Not Available

SUBMITTER: Heo S, et al.

PROVIDER: GPM32310002988 | GPMDB |

REPOSITORIES: GPMDB

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Gel-based mass spectrometric analysis of hippocampal transmembrane proteins using high resolution LTQ Orbitrap Velos Pro.

Heo Seok S Spoerk Stefan S Birner-Gruenberger Ruth R Lubec Gert G

Proteomics 20140813 17-18

Membrane proteins (MPs) play diverse important roles for physical interactions, cell communication, molecular transport, and signal transduction. Membrane proteins comprise approximately 25∼35% of the genome in living organisms, but there are difficulties in the analysis at the protein chemical level, in particular due to low abundance and limited solubility. Sequence information on membrane proteins and their complexes would be beneficial to elucidate their function. Proteins were extracted fro ...[more]

PMID: 25044505

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