Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap Velos
ORGANISM(S): Komagataella Pastoris (strain Gs115 / Atcc 20864) (yeast) (pichia Pastoris)
SUBMITTER: Akhilesh Pandey
PROVIDER: PXD000627 | Pride | 2014-11-18
REPOSITORIES: Pride
Action | DRS | |||
---|---|---|---|---|
MultiConsensus_112213.pep.xml | Pepxml | |||
MultiConsensus_PPAS_112213.prot.xml | Xml | |||
Pichia_In-Gel_1.raw | Raw | |||
Pichia_In-Gel_10.raw | Raw | |||
Pichia_In-Gel_11.raw | Raw |
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Proteomics 20141120 23-24
Pichia pastoris is a widely used eukaryotic host for production of recombinant proteins. We performed a proteogenomic analysis using high resolution Fourier transform MS to characterize the proteome of the GS115 strain. Our analysis resulted in identification of 46,889 unique peptides mapping to 3914 unique protein groups, which corresponds to ∼ 80% of the predicted genes. In addition, our proteogenomic analysis led to the discovery of 64 novel genes and correction of 11 predicted gene models. T ...[more]