Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of Sinorhizobium meliloti after exposure to elevated concentrations of the heavy metals cadmium and zinc to analyze stress responses

ABSTRACT: The stress response of the soil bacterium Sinorhizobium meliloti towards elevated concentrations of the heavy metals cadmium and zinc was analyzed via transcriptional profiling.

ORGANISM(S): Sinorhizobium meliloti

SUBMITTER: Silvia Rossbach

PROVIDER: E-MTAB-13 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Response of Sinorhizobium meliloti to elevated concentrations of cadmium and zinc.

Rossbach Silvia S Mai Danielle J DJ Carter Eric L EL Sauviac Laurent L Capela Delphine D Bruand Claude C de Bruijn Frans J FJ

Applied and environmental microbiology 20080509 13

Whole-genome transcriptional profiling was used to identify genes in Sinorhizobium meliloti 1021 that are differentially expressed during exposure to elevated concentrations of cadmium and zinc. Mutant strains with insertions in metal-regulated genes and in genes encoding putative metal efflux pumps were analyzed for their metal sensitivities, revealing a crucial role for the SMc04128-encoded P-type ATPase in the defense of S. meliloti against cadmium and zinc stress. ...[more]

PMID: 18469129

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Project description:Vibrio parahaemolyticus is a Gram-negative marine bacterium. A limited population of the organisms causes acute gastroenteritis in humans. Almost all of the clinical V. parahaemolyticus isolates exhibit a beta-type hemolysis on Wagatsuma agar, known as the Kanagawa phenomenon (KP). KP is induced by the thermostable direct hemolysin (TDH) produced by the organism, and has been considered a crucial marker to distinguish pathogenic strains from non-pathogenic ones. Since 1996, so-called âpandemic clonesâ, the majority of which belong to serotype O3:K6, have caused worldwide outbreaks of gastroenteritis. In this study, we used a DNA microarray constructed based on the genome sequence of a pandemic V. parahaemolyticus strain RIMD2210633 to examin the genomic composition of 22 strains of V. parahaemolyticus, including both pathogenic (pandemic as well as non-pandemic) and non-pathogenic strains. Over 85% of the RIMD2210633 genes were conserved in all the strains tested. Many of variably present genes formed gene clusters on the genome of RIMD2210633 and were probably acquired through lateral gene transfer. At least 70 genes over 10 loci were specifically present in the pandemic strains when compared with any of the non-pandemic strains, suggesting that the difference between pandemic and non-pandemic strains is not due to a simple genetic event. Only the genes in the 80-kb pathogenicity island (Vp-PAI) on chromosome II, including two tdh genes and a set of genes for the Type III secretion system, were detected only in the KP-positive pathogenic strains. These results strongly suggest that acquisition of this Vp-PAI was crucial for the emergence of V. parahaemolyticus strains that are pathogenic for humans. Keywords: comparative genomic hybridization, CGH Total 66 test samples were analyzed. Genomic DNA from each test strain and a reference strain (RIMD2210633) were labeled with Cy3 and Cy5, respectively, and were cohybridized on a single array. Labeling and hybridization were performed three times independently.

Dataset Information

Transcription profiling of Sinorhizobium meliloti after exposure to elevated concentrations of the heavy metals cadmium and zinc to analyze stress responses

Publications

Response of Sinorhizobium meliloti to elevated concentrations of cadmium and zinc.

Similar Datasets

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