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HeLa standard shotgun DDA analysis using a two-meter C18 monolithic silica column

ABSTRACT: Human HeLa proteome analysis by a nanoLC-MS/MS system using a two-meter C18 monolithic silica column under shallow gradient conditions without pre-fractionation was performed in this project. A hybrid quadrupole-orbitrap mass spectrometer (Thermo Q-Exactive) was used to analyze a tryptic digest of HeLa cells through this study. Identification results were filtered at 1% false discovery rate (FDR) thresholds by searching against a randomized decoy database using Mascot at peptide and protein levels.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Yasushi Ishihama

PROVIDER: PXD005159 | JPOST Repository | Mon Oct 17 00:00:00 BST 2016

REPOSITORIES: jPOST

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			Action	DRS
	150211tk04-whole_2m8h-1.raw	Raw
	150211tk04-whole_2m8h-1.wizd.mgf	Mgf
	150211tk04-whole_2m8h-2.raw	Raw
	150211tk04-whole_2m8h-2.wizd.mgf	Mgf
	150211tk04-whole_2m8h-3.raw	Raw

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