Dataset Information

MTRAQ labeled natural proteome from HeLa

ABSTRACT: To identify proteotypic peptides, HeLa cells digest labeled with mTRAQdelta0 were extensively fractionated and analyzed by LC-MS/MS.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Masaki Matsumoto

LAB HEAD: Masaki Matsumoto

PROVIDER: PXD001396 | Pride | 2016-12-07

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20111128_HeLa_mTRAQ_SCX_Fr10_bRP01.dat	Other
	20111128_HeLa_mTRAQ_SCX_Fr10_bRP02.dat	Other
	20111128_HeLa_mTRAQ_SCX_Fr10_bRP03.dat	Other
	20111128_HeLa_mTRAQ_SCX_Fr10_bRP04.dat	Other
	20111128_HeLa_mTRAQ_SCX_Fr10_bRP05.dat	Other

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Project description:In this study, we combined metabolic reconstruction, growth assays, metabolome and transcriptome analyses to obtain a global view of the sulfur metabolic network and of the response to sulfur availability in Brevibacterium aurantiacum. In agreement with the growth of B. aurantiacum in the presence of sulfate and cystine, the metabolic reconstruction showed the presence of a sulfate assimilation pathway and of thiolation pathways that produce cysteine (cysE and cysK) or homocysteine (metX and metY) from sulfide, of at least one gene of the transsulfuration pathway (aecD) and of genes encoding three MetE-type methionine synthases. We also compared the expression profiles of B. aurantiacum ATCC9175 during sulfur starvation and in the presence of sulfate, cystine or methionine plus cystine. In sulfur starvation, 690 genes including 21 genes involved in sulfur metabolism and 29 genes encoding amino acids and peptide transporters were differentially expressed. We also investigated changes in pools of sulfur-containing metabolites and in expression profiles after growth in the presence of sulfate, cystine or methionine plus cystine. The expression of genes involved in sulfate assimilation and cysteine synthesis was repressed in presence of cysteine, while the expression of metX, metY, metE1, metE2 and BL613 encoding a probable cystathionine-γ-synthase decreased in the presence of methionine. We identified three ABC transporters: two stronger transcribed during cysteine limitation and one during methionine depletion. Finally, the expression of genes encoding a methionine γ-lyase, BL929, and a methionine transporter (metPS) was induced in the presence of methionine, in conjunction with a significant increase of volatile sulfur compounds production. Refer to individual Series. This SuperSeries is composed of the following subset Series: GSE25418: BA-Methionine plus Cystine vs Cystine GSE25419: BA-Sulfate vs Cystine GSE25420: BA-Methionine plus Cystine vs Sulfate GSE25421: BA-Sulfate vs Sulfate starvation

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MTRAQ labeled natural proteome from HeLa

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