Proteomics

Dataset Information

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T74-pT74-ALLO1-PRM

ABSTRACT: To analyze phosphorylation sites of ALLO-1, worm eggs expressing GFP-ALLO-1 in the atg-11 or ikke-1 background were lysed in co-IP buffer, and GFP-ALLO-1 was immunoprecipitated with GFP-Trap-agarose beads. Proteins on the beads were directly digested with Trypsin overnight. Supernatants were collected, acidified to pH2.5 and desalted using GL-Tip SDB followed by PRM analysis.

ORGANISM(S): Caenorhabditis Elegans

SUBMITTER: Hidetaka Kosako 

PROVIDER: PXD007849 | JPOST Repository | Wed Jan 31 00:00:00 GMT 2018

REPOSITORIES: jPOST

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Publications

The autophagy receptor ALLO-1 and the IKKE-1 kinase control clearance of paternal mitochondria in Caenorhabditis elegans.

Sato Miyuki M   Sato Katsuya K   Tomura Kotone K   Kosako Hidetaka H   Sato Ken K  

Nature cell biology 20171218 1

In Caenorhabditis elegans embryos, paternally provided organelles, including mitochondria, are eliminated by a process of selective autophagy called allophagy, the mechanism by which mitochondrial DNA is inherited maternally. However, it remains unclear how paternal organelles are recognized and targeted for autophagy. Here, we identified an autophagy receptor for allophagy, ALLO-1. ALLO-1 is essential for autophagosome formation around paternal organelles and directly binds to the worm LC3 homo  ...[more]

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