Proteomics

Dataset Information

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Sensitive analysis of protein tyrosine phosphorylation


ABSTRACT: We immobilized SH2 superbinder onto a monolithic capillary column to construct a microreactor to enrich pTyr peptides from minute amount of samples. To evaluate the feasibility of this strategy, we used protein standards, phosphopeptide samples purified from pervanadate-treated HeLa cell digests as well as a protein complex sample. The results showed that this microreactor enabled highly specific enrichment of phosphotyrosine peptides.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Mingliang Ye 

PROVIDER: PXD007998 | JPOST Repository | Sat Oct 20 00:00:00 BST 2018

REPOSITORIES: jPOST

Dataset's files

Source:
Action DRS
SH2_beads_1000ug_1.raw Raw
SH2_beads_1000ug_2.raw Raw
SH2_beads_100ug_1.raw Raw
SH2_beads_100ug_2.raw Raw
SH2_beads_200ug_1.raw Raw
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