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One-step SH2 superbinder based approach for sensitive analysis of tyrosine phosphoproteome

ABSTRACT: Tyrosine phosphorylation plays a major role in regulating cell signaling pathways governing diverse biological functions such as proliferation and differentiation. Systemically mapping phosphotyrosine (pTyr) sites is the key to understand molecular mechanisms underlining pTyr-dependent signaling. Although mass spectrometry-based technologies have been widely used for pTyr site profiling and quantification, their applications are often hindered by the poor efficiency in current multi-step enrichment procedures for inherently low abundance pTyr peptides, especially under physiological conditions. Taking advantage of the sequence-independent high affinity of SH2 superbinder towards pTyr residues, we have developed a simplified one-step pTyr peptide enrichment method that uses immobilized SH2 superbinder for unbiased and robust enrichment of endogenous pTyr peptides from biological samples.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Mingliang Ye

PROVIDER: PXD012268 | JPOST Repository | Sat Mar 07 00:00:00 GMT 2020

REPOSITORIES: jPOST

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			Action	DRS
	Jurkat_one-step_1.raw	Raw
	Jurkat_one-step_1mg.raw	Raw
	Jurkat_one-step_2.raw	Raw
	Jurkat_one-step_2mg.raw	Raw
	Jurkat_one-step_3.raw	Raw

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