Dataset Information

Phosphoproteomics of Matrigel-embedded samples

ABSTRACT: Phosphoproteomics data of Matrigel-embedded HCT116 spheroid, and patient-derived colorecral cancer organoid

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Takashi Tomonaga

PROVIDER: PXD009032 | JPOST Repository | Sun Feb 24 00:00:00 GMT 2019

REPOSITORIES: jPOST

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Dataset's files

Source:

			Action	DRS
	150424_2D_Normal01_Phos_fra01.raw	Raw
	150424_2D_Normal01_Phos_fra02.raw	Raw
	150424_2D_Normal01_Phos_fra03.raw	Raw
	150424_2D_Normal01_Phos_fra04.raw	Raw
	150424_2D_Normal01_Phos_fra05.raw	Raw

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Publications

Improved phosphoproteomic analysis for phosphosignaling and active-kinome profiling in Matrigel-embedded spheroids and patient-derived organoids.

Abe Yuichi Y Tada Asa A Isoyama Junko J Nagayama Satoshi S Yao Ryoji R Adachi Jun J Tomonaga Takeshi T

Scientific reports 20180730 1

Many attempts have been made to reproduce the three-dimensional (3D) cancer behavior. For that purpose, Matrigel, an extracellular matrix from Engelbreth-Holm-Swarm mouse sarcoma cell, is widely used in 3D cancer models such as scaffold-based spheroids and patient-derived organoids. However, severe ion suppression caused by contaminants from Matrigel hampers large-scale phosphoproteomics. In the present study, we successfully performed global phosphoproteomics from Matrigel-embedded spheroids an ...[more]

PMID: 30061712

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