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A nanoLC-MS/MS system using a nonporous reverse phase column for ultrasensitive proteome analysis

ABSTRACT: The design of proteome projects is often restricted by the available amounts of sample, and thus there has been much effort to date to improve the sensitivity of proteome analyses. We have developed a new ultrasensitive nanoLC-MS system for the proteome analysis of microscale samples using a nonporous reverse phase column. Although nonporous particles have low binding capacity due to low functional group density, we found that the use of C30 particles compensated for this disadvantage and nanoLC-MS proteome analyses of microscale samples using a C30-packed column exhibited superior performance compared to porous C18 columns.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Yusuke Kawashima

PROVIDER: PXD011168 | JPOST Repository | Mon Sep 24 00:00:00 BST 2018

REPOSITORIES: jPOST

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			Action	DRS
	HEK_1000cells_T120_npC30.wiff	Wiff
	HEK_1000cells_T240_npC30.wiff	Wiff
	HEK_10ng_T120_npC30_1.wiff.scan	Wiff
	HEK_10ng_T120_npC30_2.wiff.scan	Wiff
	HEK_10ng_T120_npC30_3.wiff.scan	Wiff

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Development of a NanoLC-MS/MS System Using a Nonporous Reverse Phase Column for Ultrasensitive Proteome Analysis.

Kawashima Yusuke Y Ohara Osamu O

Analytical chemistry 20181016 21

The design of proteome projects is often restricted by the available amounts of sample, and thus there has been much effort to date to improve the sensitivity of proteome analyses. We have developed a new ultrasensitive nanoLC-MS system for the proteome analysis of microscale samples using a nonporous reverse phase column. Although nonporous particles have low binding capacity due to low functional group density, we found that the use of C30 particles compensated for this disadvantage and nanoLC ...[more]

PMID: 30350605

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