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Inhibition of T cell activation and function by the CIN85 adaptor protein

ABSTRACT: CIN85 is ubiquitously expressed adaptor protein. In T cells, CIN85 is recruited to the TCR signaling complex and inhibit TCR signaling. To reveal the molecular function of CIN85-mediated inhibition, we performed Flag-CIN85 (CIN85dA) immuno-precipitation and identified the binding proteins. As a negative control, we used deletion mutant of CIN85 (CIN85PR).

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Takashi Saito

PROVIDER: PXD012369 | JPOST Repository | Wed Jan 16 00:00:00 GMT 2019

REPOSITORIES: jPOST

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			Action	DRS
	CIN85PR_1.wiff	Wiff
	CIN85PR_1.wiff.scan	Wiff
	CIN85PR_1a_SWATH.wiff	Wiff
	CIN85PR_1a_SWATH.wiff.scan	Wiff
	CIN85PR_2.wiff	Wiff

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Publications

Inhibition of T cell activation and function by the adaptor protein CIN85.

Kong Mei Suen MS Hashimoto-Tane Akiko A Kawashima Yusuke Y Sakuma Machie M Yokosuka Tadashi T Kometani Kohei K Onishi Reiko R Carpino Nick N Ohara Osamu O Kurosaki Tomohiro T Phua Kia Kien KK Saito Takashi T

Science signaling 20190205 567

T cell activation is initiated by signaling molecules downstream of the T cell receptor (TCR) that are organized by adaptor proteins. CIN85 (Cbl-interacting protein of 85 kDa) is one such adaptor protein. Here, we showed that CIN85 limited T cell responses to TCR stimulation. Compared to activated wild-type (WT) T cells, those that lacked CIN85 produced more IL-2 and exhibited greater proliferation. After stimulation of WT T cells with their cognate antigen, CIN85 was recruited to the TCR signal ...[more]

PMID: 30723173

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