Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Proteome analysis of affinity-purified protein co-precipitates

ABSTRACT: Proteome analysis of affinity-purified materials prepared from nucleosome/chromatin fractions of HEK293T cells expressing various constructs.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Akihiko Yokoyama

PROVIDER: PXD015591 | JPOST Repository | Sat Sep 26 00:00:00 BST 2020

REPOSITORIES: jPOST

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ENL_CID.csv	Csv
	ENL_CID.raw	Raw
	ENL_CID_CID_%5BNode_07%5D.mgf	Mgf
	ENL_ETD.csv	Csv
	ENL_ETD.raw	Raw

Items per page:

1 - 5 of 20

Similar Datasets

Proteome analysis of affinity-purified protein co-precipitates

Project description:Proteome analysis of affinity-purified materials prepared from chromatin fractions of HEK293T cells expressing various constructs. HEK293T cells transiently expressing various constructs were subjected to fanChIP (fractionation-assisted chromatin immunoprecipitation) method described in our previous paper (Okuda et al. 2014 Nucleic Acids Research 42;7 p4241-4256).

2021-06-15 | PXD019783 | JPOST Repository

Proteome analysis of affinity-purified protein co-precipitates

Project description:β-adrenergic signaling-regulated MYPT1-PP1β phosphatase bridges chromatin states and actomysin tension-mediated YAP/TAZ transcriptional network for beige adipogenesis

2022-09-28 | PXD031897 | JPOST Repository

Proteome analysis of affinity-purified protein co-precipitates

Project description:HBO1-MLL interaction promotes AF4/ENL/P-TEFb-mediated leukemogenesis

2021-08-31 | PXD027400 | JPOST Repository

Proteome analysis of affinity-purified protein co-precipitates

Project description:RNA-Binding Proteins Control the Oncogenic Activity of MLL-AF4

2022-04-19 | PXD025464 | JPOST Repository

Proteome analysis of affinity-purified protein co-precipitates

Project description:Spatiotemporal Dynamics of SETD5-containing NCoR- HDAC3 Complex Determines Enhancer Activation for Adipogenesis

2021-10-22 | PXD029279 | JPOST Repository

Anti-FLAG co-immunoprecipitation of FLAG-tagged DIDO isoforms and mutants in HEK293T cells

Project description:The analysis of interactome of different DIDO isoforms and mutants upon transient overexpression of FLAG-tagged constructs in HEK293T cells.

2023-10-25 | PXD039540 | Pride

H4K20me2 SILAC Nucleosome Affinity Purification

Project description:Goal of this project was the identification of chromatin interacting proteins whose binding is differentially regulated by di-methylation of lysine 20 on histone H4 (H4K20me2). To achieve this unodified and H4K20me2-modified histone H4 were generated by native chemical ligation and assembled into recombinant di-nucleosomes. The di-nucleosomes were immobilized on streptavidin-coated beads via the biotinylated di-nucleosomal DNA and used for nucleosome affinity purifications to identify proteins regulated by H4K20me2 from SILAC-labelled HeLaS3 nuclear extracts (Arg10 and Lys8). SILAC affinity purifications were carried out in "forward" (heavy extract on modified nucleosome and light extract on unmodified nucleosome) and "reverse" (light extract on modified nucleosome and heavy extract on unmodified nucleosome) label-swap experiments and protein abundances were quantified by MaxQuant.

2019-02-27 | PXD009281 | Pride

Pull-down affinity purifications using di-nucleosomes decorated with enhancer-associated modifications and incorporating 200bp linker DNA

Project description:Characteristic features of chromatin states are not limited to particular epigenetic modifications but include other regulatory cues, such as linker DNA length, typically ranging from around 35-55 bp in most eu- and heterochromatin domains (Valouev et al, 2011; Voong et al., 2016, Cell) to over 200 bp in nucleosome-depleted regions (NDRs) found at active enhancers and promoters (Schones et al, 2008; Hansen He et al, 2010). To investigate whether and how nucleosome linker DNA affects chromatin recognition by nuclear proteins we performed an additional set of affinity purifications using di-nucleosomes incorporating different DNA linkers. Here, we investigated the effect of a 200 bp di-nucleosome linker DNA represented by scrambled DNA or SV40 enhancer DNA sequence on the nuclear protein binding to di-nucleosome decorated with enhancer-associated modifications, including H3K4me1 and H3K27ac.

2023-12-12 | PXD041443 | Pride

Identification of binding proteins to testis-specific polypeptides in testis or HEK293T cells

Project description:We prepared FLAG-tag knock-in mice for testis-specific polypeptides or transiently transfected HEK293T cells. Using these materials, we then explored the binding proteins to testis-specific polypeptides.

2021-07-06 | PXD027126 | JPOST Repository

Genome-wide maps of nucleosome positions in purified quiescent S. cerevisiae cells [MNase-Seq]

Project description:Nucleosome positions were determined for purified S. cerevisiae quiescent cells and compared to logarithmically growing cells. By analyzing nucleosome positions and MNase digestion patterns for two separate matched digestion amounts (~80% and ~50% mononucleosomes) we show that global nucleosome stabilization and repositioning occurs at transcription start sites. This repositioning indicates a repressive chromatin architecture that reflects global transcriptional shutoff in quiescent yeast.

2015-08-20 | GSE67148 | GEO