DIA proteomics of dried blood spot proteins by sodium carbonate
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo Sapiens (human)
SUBMITTER: Daisuke Nakajima
PROVIDER: PXD017185 | JPOST Repository | Mon Jan 20 00:00:00 GMT 2020
REPOSITORIES: jPOST
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SCP_5GPFs.sdia | Other | |||
| SCP_DBS.raw | Raw | |||
| SCP_DBS_GPF1.raw | Raw | |||
| SCP_DBS_GPF2.raw | Raw | |||
| SCP_DBS_GPF3.raw | Raw |
Items per page: 5 1 - 5 of 11 |
Publications
Simple and Sensitive Analysis for Dried Blood Spot Proteins by Sodium Carbonate Precipitation for Clinical Proteomics.
Nakajima Daisuke D Kawashima Yusuke Y Shibata Hirofumi H Yasumi Takahiro T Isa Masahiko M Izawa Kazushi K Nishikomori Ryuta R Heike Toshio T Ohara Osamu O
Journal of proteome research 20200604 7
Dried blood spots (DBS) are widely used for screening biomolecular profiles, including enzymatic activities. However, detection of minor proteins in DBS by liquid chromatography-mass spectrometry (LC-MS/MS) without pre-enrichment remains challenging because of the coexistence of large quantities of hydrophilic proteins. In this study, we address this problem by developing a simple method using sodium carbonate precipitation (SCP). SCP enriches hydrophobic proteins from DBS, allowing substantial ...[more]
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Study design
The urine samples (10 ml) of patients with HbA1c greater than 6.5 percent were collected in a sterile container from the endocrinology OPD, PGIMER, Chandigarh, India, after obtaining informed consent. The study was carried out following the Helsinki Declaration. The Institute Ethics Committee approved the study protocol via (IEC) vide No INT/IEC/2020/SPL-1031.
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Inclusion criteria
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The different raw files obtained are named as follows and uploaded.
1- Healthy Control
2- Diabetic Controls
3- Microalbuminuria
4- Overt albuminuria
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