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SWATH-MS proteomic analysis of liver samples prepared from liver-specific HyD-LIR expressing mice.

ABSTRACT: HyD-LIR-Venus consists of HyD, a short hydrophobic domain of Aplysia phosphodiesterase 4 short-form; LIR of TP53INP2; and a fluorescent protein, Venus. This protein efficiently binds to LC3-II and GABARAP-II localizing on inner membrane of autophagosomes and does not affect autophagosome formation.Liver samples prepared from liver-specific HyD-LIR expressing mice together with the control mice were analyzed by mass spectrometry (TripleTOF 5600) coupled to Ekspert nanoLC 415 system with Ekspert cHipLC (Sciex, Framingham, MA). The analysis results were searched against the UniProt database (release 2019_11) using ProteinPilot 5.0.2. software (Sciex). The library contained 2619 protein groups with the cutoff of 1% false discovery rate.

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Yoshiki Miura

PROVIDER: PXD019492 | JPOST Repository | Mon Nov 09 00:00:00 GMT 2020

REPOSITORIES: jPOST

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			Action	DRS
	19U315.wiff	Wiff
	19U315.wiff.scan	Wiff
	19U316.wiff	Wiff
	19U316.wiff.scan	Wiff
	19U317.wiff	Wiff

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