Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

SWATH-MS proteomic analysis of liver samples prepared from liver-specific HyD-LIR expressing mice.

ABSTRACT: HyD-LIR-Venus consists of HyD, a short hydrophobic domain of Aplysia phosphodiesterase 4 short-form; LIR of TP53INP2; and a fluorescent protein, Venus. This protein efficiently binds to LC3-II and GABARAP-II localizing on inner membrane of autophagosomes and does not affect autophagosome formation.Liver samples prepared from liver-specific HyD-LIR expressing mice together with the control mice were analyzed by mass spectrometry (TripleTOF 5600) coupled to Ekspert nanoLC 415 system with Ekspert cHipLC (Sciex, Framingham, MA). The analysis results were searched against the UniProt database (release 2019_11) using ProteinPilot 5.0.2. software (Sciex). The library contained 2619 protein groups with the cutoff of 1% false discovery rate.

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Yoshiki Miura

PROVIDER: PXD019492 | JPOST Repository | Mon Nov 09 00:00:00 GMT 2020

REPOSITORIES: jPOST

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	19U315.wiff	Wiff
	19U315.wiff.scan	Wiff
	19U316.wiff	Wiff
	19U316.wiff.scan	Wiff
	19U317.wiff	Wiff

Items per page:

1 - 5 of 20

Similar Datasets

SWATH-MS Analysis of Gfi1-mutant bone marrow neutrophils

Project description:This study investigates the proteomic alterations in bone marrow neutrophils isolated from 5-8 week old Gfi1+/-, Gfi1K403R/-, Gfi1R412X/-, and Gfi1R412X/R412X mice using the SWATH-MS technique. This dataset consists of 26 raw MS files, comprising 13 DDA (for spectral library generation)and 13 DIA (for SWATH) runs on a TripleTOF 5600 plus (SCIEX). Our findings revealed significant changes in the expression of neutrophil granule proteins and NADPH-oxidase complex components in Gfi1-mutant neutrophils.

2020-04-07 | PXD010943 | Pride

SWATH-MS Analysis of Striatal Synaptic Mitochondria from Pink1 KO rats

Project description:This study investigates the proteomic alterations in striatal synaptic mitochondria isolated from 3-month-old wild-type and Pink1 KO rats using the SWATH-MS strategy. This dataset consists of 32 raw MS files, comprising 8 DIA (SWATH) and 24 DDA runs on a TripleTOF 5600 (SCIEX). Our findings revealed synaptic mitochondrial proteomic changes due to loss of Pink1.

2016-09-28 | PXD004558 | Pride

Metabolomics analysis of mouse gut microbiota

Project description:Liquid chromatography -mass spectrometry was performed on an AB Sciex TripleTOF 5600TM mass spectrometry system (AB SCIEX, USA) for nontargeted metabolomics analysis

2022-04-24 | PXD033405 |

Untargeted metabolomics profiling of mice urine after 5 Gy and 7.5 Gy total body radiation exposure at 24 hours - LCMS Study.

Project description:Untargeted metabolomics profiling of mice urine after 5 Gy and 7.5 Gy total body radiation exposure at 24 hours. Metabolic profiling of urine samples were performed by TripleTOF 5600+ (AB Sciex) in both ionization mode

2021-11-24 | ST002165 | MetabolomicsWorkbench

Quantification of highly homologous Major Urinary Proteins in wild house mice

Project description:Male house mice were housed under semi-natural conditions and their urinary proteins were quantified using MS1 and MS2 based label-free strategies. This dataset consists of 52 raw MS files, comprising 26 DIA (SWATH) and 26 IDA runs on a TripleTOF 5600. The composition of the dataset is described in the manuscript by Enk et al., titled: "Regulation of highly homologous Major Urinary Proteins in house mice quantified with label-free proteomic methods ", Molecular Biosystems, in review.

2016-08-08 | PXD004642 | Pride

Glycine max

Project description:Soybean cultivars,Soybean Hydrophobic Protein(HPS) comparison

| PRJNA94015 | ENA

synthetic construct

Project description:genotypes of fluorescent protein variants

| PRJNA631354 | ENA

synthetic construct

Project description:Single fluorescent protein biosensor engineering

| PRJNA324720 | ENA

synthetic construct

Project description:genotypes of fluorescent protein variants

| PRJNA540481 | ENA

mCherry contains a fluorescent protein isoform that interferes with its reporter function

Project description:Fluorescent proteins are essential reporters in cell biology and molecular biology. Here, we reveal that red-fluorescent proteins possess an alternative translation initiation site that produces a short functional protein isoform. The short isoform creates significant background fluorescence that biases the outcome of expression studies. Our investigation identifies the short protein isoform, traces its origin, and determines the extent of the issue within the family of red fluorescent protein. Our analysis shows that the short isoform defect of the red fluorescent protein family may affect the interpretation of many published studies. Finally, we provide a re-engineered mCherry variant that lacks background expression as an improved tool for imaging and protein expression studies.

2022-04-19 | PXD032954 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data