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SWATH-MS Analysis of Gfi1-mutant bone marrow neutrophils

ABSTRACT: This study investigates the proteomic alterations in bone marrow neutrophils isolated from 5-8 week old Gfi1+/-, Gfi1K403R/-, Gfi1R412X/-, and Gfi1R412X/R412X mice using the SWATH-MS technique. This dataset consists of 26 raw MS files, comprising 13 DDA (for spectral library generation)and 13 DIA (for SWATH) runs on a TripleTOF 5600 plus (SCIEX). Our findings revealed significant changes in the expression of neutrophil granule proteins and NADPH-oxidase complex components in Gfi1-mutant neutrophils.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Neutrophil, Bone Marrow

DISEASE(S): Neutropenia

SUBMITTER: H. Leighton Grimes

LAB HEAD: Kenneth D. Greis

PROVIDER: PXD010943 | Pride | 2020-04-07

REPOSITORIES: Pride

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			Action	DRS
	13DDAs.zip	Other
	13DDAs_mergesearch.group	Other
	13DDAs_mergesearch__FDR.xlsx	Xlsx
	13DIAs.zip	Other
	13DIAs_SWATHprocessing.swath	Other

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Publications

Mouse models of neutropenia reveal progenitor-stage-specific defects.

Muench David E DE Olsson Andre A Ferchen Kyle K Pham Giang G Serafin Rachel A RA Chutipongtanate Somchai S Dwivedi Pankaj P Song Baobao B Hay Stuart S Chetal Kashish K Trump-Durbin Lisa R LR Mookerjee-Basu Jayati J Zhang Kejian K Yu Jennifer C JC Lutzko Carolyn C Myers Kasiani C KC Nazor Kristopher L KL Greis Kenneth D KD Kappes Dietmar J DJ Way Sing Sing SS Salomonis Nathan N Grimes H Leighton HL

Nature 20200422 7810

Advances in genetics and sequencing have identified a plethora of disease-associated and disease-causing genetic alterations. To determine causality between genetics and disease, accurate models for molecular dissection are required; however, the rapid expansion of transcriptional populations identified through single-cell analyses presents a major challenge for accurate comparisons between mutant and wild-type cells. Here we generate mouse models of human severe congenital neutropenia (SCN) usi ...[more]

PMID: 32494068

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Multiplex Biomarker Screening Assay for Urinary Extracellular Vesicles Study: A Targeted Label-Free Proteomic Approach

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2018-10-11 | PXD008891 | Pride

SWATH-proteomics analysis of ibrutinib mechanism of action in neutropenia and acute myeloid leukemia initiating mutated granulocyte-colony stimulating factor receptor

Project description:Neutrophil production and function are primarily determined by granulocyte colony stimulating factor receptor (G-CSFR). G-CSFRs associated mutations (mostly localized in the transmembrane and cytoplasmic domains of the receptor) have been reported with several distinct hematological abnormalities as well as malignancies, e.g. severe congenital neutropenia (SCN), acute myeloid leukemia (AML) and chronic neutrophilic leukemia (CNL). Ibrutinib, a small molecule Bruton’s tyrosine kinase (BTK) inhibitor, is FDA approved and clinically used against B-cell related leukemia. In our previous published work (Dwivedi et al., Leukemia.2019;33:75–87), we have shown ibrutinib’s efficacy in the mutated G-CSFRs based leukemia model systems (mouse and human). However, the signaling mechanism of ibrutinib’s efficacy is not explored yet. Here, we present a unique SWATH-based label free quantitative proteomics analysis of the normal and mutated G-CSFRs signaling post ibrutinib treatment, using 32D cell-line-based in vitro model system.

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