Dataset Information

Time course treatment with Palmitate and thapsigargin of insulinoma INS-1E cells

ABSTRACT: Time course treatment with 0.4 mM Palmitate and 200 nM thapsigargin of insulinoma INS-1E cells. Timepoints; 0, 4, 16 and 24h in biological duplicates within an iTRAQ 8 set for palmitate and thapsigargin, respectively.

ORGANISM(S): Rattus Rattus (black Rat)

SUBMITTER: Janne Lehtiö

PROVIDER: PXD020851 | JPOST Repository | Tue Aug 18 00:00:00 BST 2020

REPOSITORIES: jPOST

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	100702_VR_Palm_fr_11.raw	Raw
	100702_VR_Palm_fr_12.raw	Raw
	100709_VR_Palm_60min_Fr_13.raw	Raw
	100709_VR_Palm_60min_Fr_14.raw	Raw
	100709_VR_Palm_60min_Fr_15.raw	Raw

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Publications

Combined transcriptome and proteome profiling of the pancreatic β-cell response to palmitate unveils key pathways of β-cell lipotoxicity.

Lytrivi Maria M Ghaddar Kassem K Lopes Miguel M Rosengren Victoria V Piron Anthony A Yi Xiaoyan X Johansson Henrik H Lehtiö Janne J Igoillo-Esteve Mariana M Cunha Daniel A DA Marselli Lorella L Marchetti Piero P Ortsäter Henrik H Eizirik Decio L DL Cnop Miriam M

BMC genomics 20200826 1

<h4>Background</h4>Prolonged exposure to elevated free fatty acids induces β-cell failure (lipotoxicity) and contributes to the pathogenesis of type 2 diabetes. In vitro exposure of β-cells to the saturated free fatty acid palmitate is a valuable model of lipotoxicity, reproducing features of β-cell failure observed in type 2 diabetes. In order to map the β-cell response to lipotoxicity, we combined RNA-sequencing of palmitate-treated human islets with iTRAQ proteomics of insulin-secreting INS-1 ...[more]

PMID: 32847508

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