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Grafting induced pluripotent stem cells-derived tenocytes contribute to recovery of motor function after Achilles tendon rupture in rat

ABSTRACT: Self-renewal of tendons is rare since the vascular formation inside is extremely poor, thus reconstructive surgery using autologous tendons has been often taken place in the case of severe injury. However, the rate of re-injury after surgery is relatively high, and collection of autologous tendons leads muscle weakness which results in prolonged rehabilitation. Here, we introduce the induced pluripotent stem cells (iPSCs)-based technology aiming at developing a new therapeutic option after tendon injury. Firstly, we derived tenocytes from human iPSCs by recapitulating the normal progression of step-wise narrowing fate decisions in the vertebrate embryo. We used single-cell RNA sequencing to analyze the developmental trajectory of iPSCs-derived tenocytes. We then demonstrated that grafting iPSCs-tenocytes contributed to recovery of motor function after Achilles tendon injury in rat via engraftments and paracrine effects. The biomechanical strength of regenerated tendons recovered comparable to that of healthy tendons. We propose that the iPSCs-tenocytes will provide a novel therapeutic option in tendon injury.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Mio Iwasaki

PROVIDER: PXD020887 | JPOST Repository | Thu Jul 01 00:00:00 BST 2021

REPOSITORIES: jPOST

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			Action	DRS
	191122mi-12-Nakajima-4plex-PBS-GL7-CiRA1-DA.group	Other
	191122mi-12-Nakajima-4plex-PBS-GL7-CiRA1-DA.wiff	Wiff
	191122mi-12-Nakajima-4plex-PBS-GL7-CiRA1-DA.wiff.scan	Wiff
	191122mi-12-Nakajima-4plex-PBS-GL7-CiRA1-DA_MGFPeaklist.mgf	Mgf
	191122mi-15-Nakajima-4plex-PBS-GL7-CiRA1-DA.group	Other

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Publications

Grafting of iPS cell-derived tenocytes promotes motor function recovery after Achilles tendon rupture.

Nakajima Taiki T Nakahata Akihiro A Yamada Naoki N Yoshizawa Keiko K Kato Tomoaki M TM Iwasaki Mio M Zhao Chengzhu C Kuroki Hiroshi H Ikeya Makoto M

Nature communications 20210818 1

Tendon self-renewal is a rare occurrence because of the poor vascularization of this tissue; therefore, reconstructive surgery using autologous tendon is often performed in severe injury cases. However, the post-surgery re-injury rate is relatively high, and the collection of autologous tendons leads to muscle weakness, resulting in prolonged rehabilitation. Here, we introduce an induced pluripotent stem cell (iPSC)-based technology to develop a therapeutic option for tendon injury. First, we de ...[more]

PMID: 34408142

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