Ontology highlight
ABSTRACT:
ORGANISM(S): Homo Sapiens (human)
SUBMITTER: Yasushi Ishihama
PROVIDER: PXD027112 | JPOST Repository | Thu Oct 28 00:00:00 BST 2021
REPOSITORIES: jPOST
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Ogata Kosuke K Ishihama Yasushi Y
Molecular & cellular proteomics : MCP 20211103
Reversed-phase solid-phase extraction (SPE) techniques are commonly used for desalting samples before LC/MS/MS in shotgun proteomics. However, hydrophilic peptides are often lost during the desalting step under the standard SPE conditions. Here, we describe a simple protocol in which a stop-and-go extraction tip packed with a poly(styrene-divinylbenzene) copolymer disc is used at 4 °C during sample loading without any organic solvent. Using this method, which we designate as the CoolTip protocol ...[more]