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Antibiotic-like Activity of Atomic Layer Boron Nitride for Combating Resistant Bacteria

ABSTRACT: Amine-reactive cell surface biotinylation coupled with label-free quantification method was employed to identify the surface protein targets of BN nanosheets.

ORGANISM(S): Escherichia Coli

SUBMITTER: Mingliang Ye

PROVIDER: PXD027118 | JPOST Repository | Sun Aug 27 00:00:00 BST 2023

REPOSITORIES: jPOST

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			Action	DRS
	LYN1_HF_DDA_Ecoli2452_MassKill_AgNPs_label_bio1_20210508.raw	Raw
	LYN1_HF_DDA_Ecoli2452_MassKill_AgNPs_label_bio2_20210508.raw	Raw
	LYN1_HF_DDA_Ecoli2452_MassKill_AgNPs_label_bio3_20210508.raw	Raw
	LYN1_HF_DDA_Ecoli2452_MassKill_AgNPs_label_bio4_20210508.raw	Raw
	LYN1_HF_DDA_Ecoli2452_MassKill_AgNPs_unlabel_bio1_20210508.raw	Raw

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Antibiotic-like Activity of Atomic Layer Boron Nitride for Combating Resistant Bacteria

Project description:The rapid global rise of antimicrobial resistance (AMR) that increasingly invalidates conventional antibiotics has become a huge threat to human health. Although nanosized antibacterial agents have been extensively explored, they cannot sufficiently discriminate between microbes and mammals, which necessitates the exploration of other antibiotic-like candidates for clinical uses. Herein, two-dimensional boron nitride (BN) nanosheets are reported to exhibit antibiotic-like activity to AMR bacteria. Interestingly, BN nanosheets had AMR-independent antibacterial activity without triggering secondary resistance in their long-term use and displayed excellent biocompatibility in mammals. Surface proteome analysis coupled with molecular dynamic simulations and Bio-Layer Interferometry revealed that BN nanosheets could rapidly interact with the key surface proteins of cell division including FtsP, EnvC, and TolB, resulting in a specific antibacterial mechanism by impairment of Z-ring constriction in cell division. Notably, BN nanosheets had a potent antibacterial effect in a lung infection model by P. aeruginosa (AMR), displaying a two-fold increment of survival rate. Overall, these results suggested that BN nanosheets could be a promising nano-antibiotic to combat resistant bacteria and prevent AMR evolution.

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Detecting Tumor Specific Antigen-Reactive T cells from Tumor Infiltrating Lymphocytes via Interaction Dependent Fucosyl-biotinylation [TCR-seq]

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Project description:To understand how pluripotency is controlled, we employed a modification of standard ChIP-chip method (biotin-mediated ChIP-chip) to identify targets of nine factors, including somatic cell reprogramming factors (Oct4, Sox2, Klf4, c-Myc) and others (Nanog, Dax1, Rex1, Zpf281, and Nac1) in mouse ES cells. We show the application of In vivo biotinylation mediated ChIP (bioChIP) to global target mapping. Our data also show that differential regulation of target genes correlates with the extent of promoter occupancy by multiple factors in maintaining stem cell pluripotency. Keywords: Biotin-mediated Chip-chip, Antibody ChIP-chip, Mouse embryonic stem cells

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| S-EPMC6720579 | biostudies-literature

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