Proteomics

Dataset Information

0

A stress-reduced passaging technique improves the viability of human pluripotent cells

ABSTRACT: Xeno-free culture systems have expanded the application of human pluripotent stem cells (PSCs). Here we describe an improved method for the subculture of human PSCs. The revised method significantly facilitated the viability of human PSCs by lowering DNA damage and apoptosis, resulting in more efficient and reproducible downstream applications. Furthermore, the method did not alter the PSC characteristics after long-term culture and attenuated the growth advantage of abnormal subpopulations. This robust passaging method minimizes experimental error and improves the quality control of human PSC research and applications.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Mio Iwasaki 

PROVIDER: PXD028731 | JPOST Repository | Sun Jan 23 00:00:00 GMT 2022

REPOSITORIES: jPOST

Json Xml

Dataset's files

Source:
Action DRS
210823mi-29-WTB6-P50-250ng-DIA_Slot1-01_1_6431.tdf Other
210823mi-29-WTB6-P50-250ng-DIA_Slot1-01_1_6431.tdf_bin Other
210823mi-30-WTB6-P51-250ng-DIA_Slot1-25_1_6432.tdf Other
210823mi-30-WTB6-P51-250ng-DIA_Slot1-25_1_6432.tdf_bin Other
210823mi-31-WTB6-P52-250ng-DIA_Slot1-49_1_6433.tdf Other
Items per page:
1 - 5 of 20
altmetric image

Publications

A stress-reduced passaging technique improves the viability of human pluripotent cells.

Takahashi Kazutoshi K   Okubo Chikako C   Nakamura Michiko M   Iwasaki Mio M   Kawahara Yuka Y   Tabata Tsuyoshi T   Miyamoto Yousuke Y   Woltjen Knut K   Yamanaka Shinya S  

Cell reports methods 20220124 2

Xeno-free culture systems have expanded the clinical and industrial application of human pluripotent stem cells (PSCs). However, reproducibility issues, often arising from variability during passaging steps, remain. Here, we describe an improved method for the subculture of human PSCs. The revised method significantly enhances the viability of human PSCs by lowering DNA damage and apoptosis, resulting in more efficient and reproducible downstream applications such as gene editing and directed di  ...[more]

Similar Datasets

Transcriptomics A stress-reduced passaging technique improves the viability of human pluripotent cells
2022-01-23 | GSE184071 | GEO
Transcriptomics Suspended hydrogel culture as a method to scale up intestinal organoids
2023-09-11 | GSE226539 | GEO
Transcriptomics Expression data from human adipose stem cells expanded in allogeneic human serum and fetal bovine serum
2010-04-01 | GSE17090 | GEO
Transcriptomics Impact of CD4+ T cell help on CD8+ CAR T cell gene expression during ex-vivo manufacturing
2023-11-30 | GSE245427 | GEO
Transcriptomics Expression data from human adipose stem cells expanded in allogeneic human serum and fetal bovine serum
2010-04-13 | E-GEOD-17090 | biostudies-arrayexpress
Transcriptomics Chemically defined and scalable culture system for intestinal stem cells derived from human intestinal organoids (scRNA-Seq)
2023-11-20 | GSE219017 | GEO
Transcriptomics Chemically defined and scalable culture system for intestinal stem cells derived from human intestinal organoids (RNA-Seq)
2023-11-20 | GSE219016 | GEO
Transcriptomics Baffled-flow culture system enables the mass production of megakaryocytes from human embryonic stem cells by enhancing mitochondrial function
2023-12-14 | GSE228163 | GEO
Transcriptomics Efficient Expansion and Delayed Senescence of Human Umbilical Cord Mesenchymal Stem Cells by Microcarrier-Bioreactor System
2023-09-13 | GSE233634 | GEO
Transcriptomics Expanding highly homogenous population of human primordial germ cell like cells in long-term and feeder-free culture condition [RNA-Seq]
2021-05-15 | GSE174465 | GEO