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Digestion time course of human liver plasma membrane

ABSTRACT: The selection of signature peptides is critical for reliable peptide quantification. The purpose of this study was to comprehensively assess the digestion efficiency and stability of tryptic peptides and to identify optimal signature peptides for human hepatic transporters and membrane marker proteins. The plasma membrane fraction of the human liver was digested at different time points (0.5, 1, 2, 4, 8, 16, 24, and 48 h) and the peptides were comprehensively quantified using quantitative proteomics.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Sumio Ohtsuki

PROVIDER: PXD034402 | JPOST Repository | Wed Oct 12 00:00:00 BST 2022

REPOSITORIES: jPOST

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			Action	DRS
	IDA_1.wiff	Wiff
	IDA_1.wiff.scan	Wiff
	IDA_2.wiff	Wiff
	IDA_2.wiff.scan	Wiff
	IDA_3.wiff	Wiff

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Publications

Human Hepatic Transporter Signature Peptides for Quantitative Targeted Absolute Proteomics: Selection, Digestion Efficiency, and Peptide Stability.

Mori Ayano A Masuda Takeshi T Ito Shingo S Ohtsuki Sumio S

Pharmaceutical research 20220921 11

<h4>Purpose</h4>Quantitative targeted absolute proteomics (QTAP) quantifies proteins by measuring the signature peptides produced from target proteins by trypsin digestion. The selection of signature peptides is critical for reliable peptide quantification. The purpose of this study was to comprehensively assess the digestion efficiency and stability of tryptic peptides and to identify optimal signature peptides for human hepatic transporters and membrane marker proteins.<h4>Methods</h4>The plas ...[more]

PMID: 36131112

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